FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1912-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1912-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70009
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGTATCTGCGATGGGATTAGAATTTTCAACTAGGATGAATGTTGAG3130.44708537473753374No Hit
GTATCTGCGATGGGATTAGAATTTTCAACTAGGATGAATGTTGAGCATTG2900.414232455827108No Hit
CATCCATAAAATGGGCCTATGATATAACCTACCTCCTTCAGTGGGGGAGG2840.40566212915482297No Hit
CTGTATGGCTGTGGGCTGTTTGCTTAGCTTTTCTGAACCCCTATTACCTC2780.397091802482538No Hit
GTAGAAACAAGGTACGGTATCTGCGATGGGATTAGAATTTTCAACTAGGA2490.3556685568998272No Hit
GTCTTATATCAGAGGAGGTGAAGCCACACTGGCATCTTATAAGTATATGG2410.34424145467011386No Hit
AGTAGAAACAAGGTACGGTATCTGCGATGGGATTAGAATTTTCAACTAGG2290.32710080132554387No Hit
AAACAAGGTACGGTATCTGCGATGGGATTAGAATTTTCAACTAGGATGAA2180.31138853575968806No Hit
GCTGTGGGCTGTTTGCTTAGCTTTTCTGAACCCCTATTACCTCATCCATA2180.31138853575968806No Hit
GATATAACCTACCTCCTTCAGTGGGGGAGGACCACATTTTAAGAATGTGG2080.2971046579725464No Hit
GTATATGGGGACCCACTTTCTTCCTGGTGACCACTAATTTTCCTTTGCTG1980.28282078018540474No Hit
AAGCAGGACAGGGAGAAGCAGGTTTCCTATTGACCAGGGAGCCCGATGTG1800.2571098001685498No Hit
CTATTACCTCATCCATAAAATGGGCCTATGATATAACCTACCTCCTTCAG1710.24425431016012228No Hit
CCTATTACCTCATCCATAAAATGGGCCTATGATATAACCTACCTCCTTCA1710.24425431016012228No Hit
ATATAACCTACCTCCTTCAGTGGGGGAGGACCACATTTTAAGAATGTGGT1680.2399691468239798No Hit
GTATGGCTGTGGGCTGTTTGCTTAGCTTTTCTGAACCCCTATTACCTCAT1620.2313988201516948No Hit
GGATTAGAATTTTCAACTAGGATGAATGTTGAGCATTGTCACCACTCAGA1610.2299704323729806No Hit
AGGTACGGTATCTGCGATGGGATTAGAATTTTCAACTAGGATGAATGTTG1550.22140010570069563No Hit
GGTTTTGCCTTTGCCATTTATTTTCTGTATGGCTGTGGGCTGTTTGCTTA1520.21711494236455312No Hit
AATCCAGGTTTTGCCTTTGCCATTTATTTTCTGTATGGCTGTGGGCTGTT1400.19997428901998315No Hit
CTTTAGAAAAGAGGAGGGATGCCTGTCTGGCTTAGTTGGTAGAGAATATG1390.198545901241269No Hit
TTTATTTTCTGTATGGCTGTGGGCTGTTTGCTTAGCTTTTCTGAACCCCT1380.1971175134625548No Hit
GTCAAATCCAGGTTTTGCCTTTGCCATTTATTTTCTGTATGGCTGTGGGC1380.1971175134625548No Hit
GGTACGGTATCTGCGATGGGATTAGAATTTTCAACTAGGATGAATGTTGA1350.1928323501264123No Hit
AAAGCAGGGAGAGCAGCAGGCAGGAGGAGAAGCAGGGTCCCCACTGAGCA1330.18997557456898398No Hit
GTATTTGCCTTTCATACTCCCTTGGTTTTATCATCCTTGGAAATAGTCTC1320.18854718679026983No Hit
GATGAATGTTGAGCATTGTCACCACTCAGAGGGCATTTGTCTTATATCAG1300.1856904112328415No Hit
ATGTTGAGCATTGTCACCACTCAGAGGGCATTTGTCTTATATCAGAGGAG1240.1771200845605565No Hit
TCCCTAGTCTGACCTCACTCTCTCGTTTCAAAAGTAACCAATGTCTTAGT1190.16997814566698566No Hit
GGATGAATGTTGAGCATTGTCACCACTCAGAGGGCATTTGTCTTATATCA1180.1685497578882715No Hit
GTATTGAATTGTTCTCTAAAATAGCTATACCAGGGACTCCTGAGTGGCTG1170.16712137010955735No Hit
CGGTATCTGCGATGGGATTAGAATTTTCAACTAGGATGAATGTTGAGCAT1100.15712265565855818No Hit
GTACATGAAAGTGCTGAAGCAAAATGCCATTAATAGTCCTGGTATTATTA1050.14998071676498734No Hit
TGATATAACCTACCTCCTTCAGTGGGGGAGGACCACATTTTAAGAATGTG1030.14712394120755903No Hit
CAAGGTACGGTATCTGCGATGGGATTAGAATTTTCAACTAGGATGAATGT1030.14712394120755903No Hit
CATTTTAAGAATGTGGTACATGAAAGTGCTGAAGCAAAATGCCATTAATA980.1399820023139882No Hit
GATTAGAATTTTCAACTAGGATGAATGTTGAGCATTGTCACCACTCAGAG970.13855361453527404No Hit
TCTTATAAGTATATGGGGACCCACTTTCTTCCTGGTGACCACTAATTTTC960.13712522675655986No Hit
GGACAGGGAGAAGCAGGTTTCCTATTGACCAGGGAGCCCGATGTGTGGCT950.1356968389778457No Hit
GAAACAAGGTACGGTATCTGCGATGGGATTAGAATTTTCAACTAGGATGA950.1356968389778457No Hit
GTAAATAGGTGAATATGTCCAAATAACCAAATCAGCCATAAAAGTAGTTC940.13426845119913156No Hit
CCCTTGGTTTTATCATCCTTGGAAATAGTCTCATGACAGCTAAGTGAAGA920.1314116756417032No Hit
AGCGAAAGCAGGGTCAAATCCAGGTTTTGCCTTTGCCATTTATTTTCTGT910.12998328786298904No Hit
GGGCTGTTTGCTTAGCTTTTCTGAACCCCTATTACCTCATCCATAAAATG870.12426973674813238No Hit
AGCAGGGGGAGGGCAGAGGGAGAGGAAAACAATCCTCAAGCAGGGAGCCT840.1199845734119899No Hit
GTCACCACTCAGAGGGCATTTGTCTTATATCAGAGGAGGTGAAGCCACAC830.11855618563327572No Hit
AAAGCAGGCTGTGAAAGGAGCAAGAGGCTAAGAAGCTGCTCCGCCCTCAA790.11284263451841905No Hit
GGTCAAGACTCTGTGATAAACCAGAAATGGTCCACATGAAATTTACCAGA780.1114142467397049No Hit
GAATATATTCTGTTGAATTAACAGCGAGCAAATTTTAAACCACAGAACCA780.1114142467397049No Hit
ATCTGCGATGGGATTAGAATTTTCAACTAGGATGAATGTTGAGCATTGTC770.10998585896099074No Hit
GGCTTATAGTGGTTAAATACCTCATCTGAGGCCACGTAGCTGCTCACAAC760.10855747118227656No Hit
CTCAGAGGGCATTTGTCTTATATCAGAGGAGGTGAAGCCACACTGGCATC740.10570069562484824No Hit
GTAGATAAAAGTGCACACGAGGGATGAGAACATTAATTACATCCAAAGAA730.10427230784613407No Hit
CTTATAAGTATATGGGGACCCACTTTCTTCCTGGTGACCACTAATTTTCC720.1028439200674199No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTAAA100.0070468863145.206011
ACACCGG100.007077091144.998576
CACCGGG100.007077091144.998577
CCTAGTC301.09099346E-7120.8321463
AGCGAAA1250.098.740091
CACGAGC259.3504245E-486.999146145
ATAGGTG353.415114E-582.856325
TCTGACC451.2209748E-680.554768
GAGCGAA554.2922693E-879.203281
GCGAAAG1650.074.696232
GTCTGTA300.001928279372.499299
CCCTAGT502.2840104E-672.499292
AGACAGC300.001928279372.499297
AGTAGAA1250.069.698881
TCCCTAG553.9885017E-666.002731
CGAAAGC1900.064.867783
AATAGGT451.186205E-464.443814
GTAACAA350.003532719862.2311441
CTTATAG606.740309E-660.4160733
TAGGTGA501.9973952E-457.999436
Produced by FastQC (version 0.11.5)