FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1912-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1912-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727074
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT8570.11786970789768303No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA7870.10824207714758058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT805.8714613E-754.3751
GTACTAC450.00963808848.3333323
TTACGTG1053.8226754E-641.428572
GTAGAAA5800.038.752
GATATAA800.002036250236.252
TATACTG6700.035.7089545
AAGGTCG1751.0085569E-733.1428579
TACAGGG2653.6379788E-1232.830192
TTATACT5850.032.222224
TATAGAC1851.6425656E-731.3513493
GTAATAT2351.2605597E-930.8510651
GTATACG950.00472645730.5263141
TATAATA4550.030.2747272
GATATAG2252.8148861E-829.0000021
ACAAGGC6850.028.5766418
CCTTATA6150.028.2926852
AGCGAAA3102.7284841E-1128.0645161
CTAGTAC1050.00770708927.6190473
GTTGACA4850.026.9072171
TAGAAAC9700.026.9072173
Produced by FastQC (version 0.11.5)