FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1912-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1912-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367663
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT75732.059766688516386No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT64701.7597636966461132No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG61021.6596720366204922No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG60551.646888590910698No Hit
GCTTTAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT55391.506542676309555No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA51601.403459146011429No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA47821.300647603919894No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC37621.0232196331967047No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG35270.9593024046477345No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA31020.8437074168464056No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA29130.7923016458006381No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC23980.6522277194060866No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG22410.6095255709712427No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC22250.6051737596657809No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCC21270.5785189154198274No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG20910.5687273399825383No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG20690.5627435994375284No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA19900.5412565311168108No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG19410.527929108993834No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA18630.5067140288797077No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG18040.49066672469081746No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT18000.4895787718644519No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT17310.470811585609648No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG17080.4645558568580467No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG16710.45449229321416623No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTT15720.4275654607616214No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTC15590.42402961407593365No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG15560.4232136494561596No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT14880.404718451407947No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT14640.3981907344497542No Hit
GGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAA14570.39628681700361473No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTA13900.37806360716199344No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT13800.3753437250960798No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC13460.3660961260719735No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA13430.36528016145219944No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA13330.3625602793862858No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACT12780.34760092802376086No Hit
TCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACT11990.3261138597030433No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC11740.31931415453825923No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT11220.3051707677955084No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC11100.30190690931641206No Hit
ATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGG11060.3008189564900466No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT10780.29320328670548845No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG10770.2929312984988971No Hit
ATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAA10580.2877635225736612No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG10360.2817797820286512No Hit
GGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATAT10340.2812358056154685No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA10270.27933188816932897No Hit
GTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGA10230.27824393534296354No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA10200.2774279707231894No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT9610.26138066653429903No Hit
GGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGA9510.2586607844683854No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG9300.25294903212996683No Hit
GACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACACATCAG9270.25213306751019277No Hit
CCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCTCAG9080.24696529158495686No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA9000.24478938593222596No Hit
ATGCAATAAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCAT8930.24288546848608644No Hit
GCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTT8490.23091798739606648No Hit
GGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCG8370.22765412891697015No Hit
CTCTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCT8310.226022199677422No Hit
GGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCAT8180.22248635299173428No Hit
GGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTG8120.22085442375218609No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG7930.2156866478269502No Hit
GTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAAT7930.2156866478269502No Hit
CAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA7890.2145986950005848No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTCAGCTGAAA7820.21269477755444527No Hit
ATACTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAAGTACAC7590.2064390488028439No Hit
GAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATACTGACAAA7500.20399115494352166No Hit
GAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAAAAT7440.2023592257039735No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGA7410.2015432610841994No Hit
CTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAG7400.20127127287760802No Hit
CTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCT7360.2001833200512426No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATC7310.19882337901828578No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG7210.1961034969523721No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA7190.1955595205391894No Hit
TTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACA7090.1928396384732758No Hit
GCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGC6980.18984776820077082No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA6970.18957577999417946No Hit
ATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCA6890.18739987434144856No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCA6830.18576794510190037No Hit
AGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGAT6800.1849519804821263No Hit
ATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAAAATGCAATA6760.18386402765576085No Hit
CCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACAG6750.18359203944916946No Hit
GTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTAT6720.1827760748293954No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG6700.18223209841621266No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG6590.17924022814370769No Hit
GTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCC6510.17706432249097678No Hit
ATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTG6360.17298449939210636No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT6310.17162455835914953No Hit
TGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTT6270.17053660553278407No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGTCTCCTTGC6270.17053660553278407No Hit
CAATGAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCT6270.17053660553278407No Hit
AACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAG6180.16808871167346184No Hit
GATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTC6000.16319292395481733No Hit
GAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAA5950.16183298292186052No Hit
GGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAAGA5910.16074503009549507No Hit
ATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGATGCTT5910.16074503009549507No Hit
TCAATGAACTGAGCAATCTTACGAAAGGGGAGAAAGCTAATGTATTGATA5880.15992906547572097No Hit
ATGGAAGACTTTGTGCGACAGTGCTTTAATCCAATGATTGTCGAGCTTGC5880.15992906547572097No Hit
GTGGATCATATGGCCATAATTAAGAAGTACACATCAGGAAGACAGGAGAA5870.15965707726912962No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTC5850.15911310085594688No Hit
TTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGTC5780.15720918340980736No Hit
CTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGT5470.14877754900547513No Hit
AAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATGGCCTCCATCAT5440.14796158438570103No Hit
TTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAA5400.14687363155933558No Hit
ACTTTACTTTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATT5320.1446977259066047No Hit
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATA5310.14442573770001332No Hit
TAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATACTG5310.14442573770001332No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGAT5260.14306579666705652No Hit
GATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGA5230.14224983204728245No Hit
CCCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACA5220.14197784384069106No Hit
CGCGAGATACTGACAAAAACCACTGTGGATCATATGGCCATAATTAAGAA5200.14143386742750835No Hit
CCCCCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAA5160.1403459146011429No Hit
CCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACA5160.1403459146011429No Hit
GCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA5100.1387139853615947No Hit
ATGAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGC5000.1359941032956811No Hit
TCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCA4960.13490615046931564No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA4870.1324582566099934No Hit
ATCTCGCACTCGCGAGATACTGACAAAAACCACTGTGGATCATATGGCCA4820.13109831557703658No Hit
TAATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGA4770.12973837454407977No Hit
TCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCTCA4730.12865042171771432No Hit
TGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATC4710.1281064453045316No Hit
CAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCA4710.1281064453045316No Hit
TATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGA4670.12701849247816616No Hit
TTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATG4650.12647451606498342No Hit
ATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGA4600.1251145750320266No Hit
GCTTCATGTATTCAGACTTCCATTTCATTGACGAACGAGGCGAATCAATA4580.12457059861884388No Hit
GAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGA4560.12402662220566117No Hit
CACTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAA4480.12185071655293027No Hit
TCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGA4440.12076276372656482No Hit
GAGTAGTAGTTTTCTCGATTCAGCTGAAAATCCTTCGAGTTGTGGAGATG4440.12076276372656482No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC4400.11967481090019937No Hit
GAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCC4360.11858685807383393No Hit
ACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGC4330.11777089345405983No Hit
ATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAA4330.11777089345405983No Hit
ACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGG4320.11749890524746848No Hit
GATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAAAATGCAAT4300.11695492883428574No Hit
AAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTT4300.11695492883428574No Hit
CTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACTG4200.11423504676837212No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACTACGT4170.11341908214859804No Hit
CTACGTCTCCTTGCCCTATCAATACATTAGCTTTCTCCCCTTTCGTAAGA4170.11341908214859804No Hit
ATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCC4120.11205914111564123No Hit
TTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAG4120.11205914111564123No Hit
AGACAATGAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTC4110.11178715290904988No Hit
CAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAG4090.11124317649586714No Hit
GTATAAGGGAATGTGGTACTTATTGCATTTTGCGCTGGCACTTTTAAGAA4070.11069920008268443No Hit
CAATCCGACTTTACTTTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTA4060.11042721187609304No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTTAATCCAATGA4040.10988323546291033No Hit
CCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGGGAGGTGG4030.10961124725631897No Hit
TAAAAGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACTGGAGA4000.10879528263654488No Hit
GAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAA4000.10879528263654488No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTACGAAAGGGGAGAAAGCTAA3980.10825130622336215No Hit
AGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACT3970.1079793180167708No Hit
ATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAG3790.10308353029812628No Hit
ATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCT3730.1014516010585781No Hit
CTGCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCAT3720.10117961285198675No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATA100.007089072144.99986145
AGTAGAA12200.0112.330741
AGCGAAA1700.0106.632041
TATTAGT553.6379788E-12105.468781
GCAGGTA8450.0104.674462
CAGGTAC8650.0102.2542343
GGTACTG9150.096.666585
AGCAGGT9350.094.611721
GGTCAAA2800.090.637241
GCGAAAG2050.088.414552
AGTTTTA502.2646418E-886.999916145
ACTAATA703.092282E-1182.857068
ACTGATC10700.082.6634758
TACTGAT10800.081.898077
GTCAAAT3100.081.854772
GTACTGA10850.081.520676
CGAAAGC2250.080.555483
GTTAGTT451.2313376E-680.55548145
CTGATCC11150.079.3272869
ACAAGGC12450.075.702748
Produced by FastQC (version 0.11.5)