FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1911_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1911_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595801
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG12220.20510203910366043No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT9620.16146330737947737No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA9260.15542102144843664No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC9220.1547496563449877No Hit
GTTTTCTTCTTGTAATTGATTTCTAGTTCTATACCACTGTGGTTGGAAAA7770.13041267134496248No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG7190.12067787734495242No Hit
GTTGACATGGTCCTATGATGTTGATCCTTCGTTTTGTGAATGCGGCCCAT6980.11715321055184533No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT6050.10154397189665676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG1600.054.374912
CGAAAGC1750.049.71423
GTTACGT901.320388E-648.345421
AGCGAAA1900.045.8009221
GTGATCG855.338938E-542.6469889
TTACGTG855.338938E-542.6469882
TAGTCGG907.47887E-540.2777068
GCAGTGC5750.036.5743641
CAGTGCA5600.036.2499352
CAACCCG2551.8189894E-1234.117584145
CAGCAAC4400.031.306767
GTGCAGC7100.030.633754
AGTGCAG6800.029.8528923
CTAGTAC1000.00607256528.999953
GCTACTA2252.8130671E-828.9999499
AGTCGGT1306.479916E-427.8845679
TATACTG2354.2915417E-827.765915
GTTGACA6800.026.6610771
ACATGGT7400.026.4526545
CCTATAT1100.00965673126.370231
Produced by FastQC (version 0.11.5)