FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1911_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1911_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTTACTTATCCCTGACAGAGGCTAGCACTAGCTGGGAACTTCTTCTA10.6172839506172839No Hit
TCCCCGGCCCCTGGGATCCTGACCTGAGAGGGGAGACACTTAATGACTGA10.6172839506172839No Hit
TCCCAATAGTGGGGGCTTCCCGGGACAAACAGGGGCAATCTGGGCCCATC10.6172839506172839No Hit
GCTACAAGGGATCCATGTTCAAAGACCAGGGGGTGGACGGTAGTACTGTG10.6172839506172839No Hit
GAGAGAAAAGAGGAGAGAGGAATGGAGTCAGCCCGTGAGGTGGGGGGATT10.6172839506172839No Hit
GATTTTATTTATTTATTTGACAGAGAGAGATCACAAGTAGGCAGAGAGGC10.6172839506172839No Hit
CTCATTAACTACTGTTCTCCACATTTCAGAGAGGGGCCCAGAGCCCACAG10.6172839506172839No Hit
ATGGCTGACTCAAAAGAGCGGTTTTTAAAATGTTAAAGACGCCCAATCCA10.6172839506172839No Hit
CTTGTGACCTGCTGTTGGGAGGAAGAGAATGGTCCTACATCGTCGAAAGA10.6172839506172839No Hit
GGATCAGGAGATCTGCGTAAACACCTCCCATGAGCTGCCCTTCCTTTGTC10.6172839506172839No Hit
AAAGCAGGGTGTGCTGAAAGGGGTGGAGGGGGTGCTTTAAAGGGAGGTGG10.6172839506172839No Hit
CTTCCAAATAGCTCGATATCGGCCCCGGGGGCACTAGCATCTCAGAAACA10.6172839506172839No Hit
GCCCCCTCCTTGGACCTGGACCCGGGGCAAACAGAGATTCTCTGGCTGCC10.6172839506172839No Hit
TAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATACTG10.6172839506172839No Hit
GCCGCCGTCCCCTGCCCTCCTGGTGCCGGGAATGGCCCTTGGGCCTCCAA10.6172839506172839No Hit
GTTTTATACCAGGGCTCATCCCTAATTAGCACAGATGCCTGAAGTATGTG10.6172839506172839No Hit
GCTTGGAGGACAGTCATATGTTCATTGGACCGTATGCAGAATTCCAACGA10.6172839506172839No Hit
GTAGATGTGGATTCATCAGTTCTGGGGGAATCCTGCCTTCTTTTATTAAT10.6172839506172839No Hit
GATGGAGCTTACTTTCTAGTGGGAGGGCTTAAGTTCTGGTAGGGGACATA10.6172839506172839No Hit
CCGTAAGGGAGTGCCAGCAAAAGGCCTCACACGTTCCAAATGTCAGTAAA10.6172839506172839No Hit
ATCCAGGAAGGACTTCTTGAAGTTAACTTTTGAGCAGAGCCCTGAAGGAG10.6172839506172839No Hit
ATCCCAGATAAGTGGGATCAGACAGTATTTGTCCGTTTGTATCAGGGTGA10.6172839506172839No Hit
TAATAATGCTGATCGCCTAGGGTGTTGCCAGGATTCAAGGAGCACCTTGA10.6172839506172839No Hit
CTATCCGACTCGCCCAGAAGGTCTACCTGTCCAACCTGACACTGCAGCAC10.6172839506172839No Hit
GAATATGGCACCGGAAAAATTGGACTTTGAGGACTGCAAAGATATTGGCG10.6172839506172839No Hit
CTTTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTA10.6172839506172839No Hit
GCAAAGGCTCTTAAGAATGTACTATGAACAATTACACCCCAACAAGCTGG10.6172839506172839No Hit
GTGGCGCTGGAGACACACAGCTGTAATGCCAAACATCAGTGATTTCATTT10.6172839506172839No Hit
CATTTTTGTCTGTGGATGATTTGTAGAATTCACTGGTGAAACCATGGGGT10.6172839506172839No Hit
GGATATTAAAAATAACGAGACAAGAGCATCATGGGCGACTGGAGATTCAT10.6172839506172839No Hit
CTTCCATGGCTTCTGCTGCCTGCTCACTTGATCCAGCCATCTGCTCCATG10.6172839506172839No Hit
CTCTTACTGTATTGTAGAATTTGGTTTGCAGATATTTGGGTGAGGATTTT10.6172839506172839No Hit
GCAAAAAATTTCCCAAAATGTCAACCAAAAAAGTCTGCCTTGGATATATT10.6172839506172839No Hit
GATATATACCCAGCAGCAAAATTGGTGAGTCATATAGTAGTTCTCCTTTT10.6172839506172839No Hit
ACATGGAGAAGAACCAGCTCGAAGAGGTGCCCTCGGCCCAGCCCCGGCAC10.6172839506172839No Hit
CCCCATGGCAGCCCCCCTTGTGCCTGTGCAACAAGGCCCACAAACAGTCC10.6172839506172839No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC10.6172839506172839No Hit
CAATTATTTCATATTGCTTGTTCATCTTGTCGACTATATTATTCACCTTG10.6172839506172839No Hit
TTGCTGCCCTCTGGCTTATGGACTTCATGAGCCCCAGTTGCCTCCCTTGT10.6172839506172839No Hit
GTCTGTCTGTCTCTCTCCCCAGCTCTGTGTCAAATAAATAAATGGCATCT10.6172839506172839No Hit
GGGCATGTAAGGCTCTCCCAAAGAAGAGGTGTTCCTGGGGGCATTTCAGT10.6172839506172839No Hit
CTTTTCTATGAATTAAACACATAGGGTAGCACTTGAGAAGAACGGTGAAG10.6172839506172839No Hit
GTTTTATGGGGATATAGCTTCAGTTTTCAAGAAGAAAATCCTTCCGGAGA10.6172839506172839No Hit
CCCTCTGGCTGACCACATCTGTGGGGTGTATGGATGTGTGTGAGTGTGTG10.6172839506172839No Hit
CTTCGTTCGCCAGTTGAGAAAGATTGACGTCAATGCAGCCGGTGGGTTTT10.6172839506172839No Hit
GGGGGGGCCCCGGGGGGGCCGGGGGAGGCCACGGCAGATCAGGACGTCCC10.6172839506172839No Hit
GGAGGGAGAATGATTAAGTGGGAGATCTGCGTGGACGAGTGCCTCCGTGC10.6172839506172839No Hit
TCCCAGTAGTGTGACCCCACTTTCCATAGCTGGAGAATGTATTCATCACT10.6172839506172839No Hit
GTTTTAAGATGGCCCCAGTTCCTTTTACCAGTCTGTTGGTGACCACAGAA10.6172839506172839No Hit
GTGTAAGTCACATTCCAGGTTGTGTCTGGGAAGATTTGGATTCTTTGGTA10.6172839506172839No Hit
CCCATACACAGAGGATCAGAGAGGCTTAGAACTAGCTCATCTCTCCAGAG10.6172839506172839No Hit
TCCCTCTGCGGAGGCTCTGAAACCTTCCTTCCTTGCCGTCACTCCTGGAT10.6172839506172839No Hit
GTGATAAAGACTTGTCTCAGTGAATCTTCAAAACAACCTGATAAGGTGGG10.6172839506172839No Hit
GGGCCAGGGATACTGGCGCCTTGGAGATCGCAGTCTGGCCAGGGAGAGGC10.6172839506172839No Hit
CAGGAGCACCACGCAGGAGCCGGTAACAGCTGCCGGGGGGCGGGGGGCAG10.6172839506172839No Hit
GCCTGAGTTTGGGGCTGGGCCTGGGCCTGGGTCTTGGTCGCCTTGTCAGC10.6172839506172839No Hit
GGCGAGGTTAGGAAAAGGATACATGTTCGAGAGTAAGAGCATGAAGCTAC10.6172839506172839No Hit
GCTGATAAGGAAGCCCCTGGACGGGCGGTGACCATGCTGGGCCCAAGGAC10.6172839506172839No Hit
GGACAGGTATCATCTCCCTTGAATAGGTGGGAGACAGAGGTCCAATGAGG10.6172839506172839No Hit
GCCAGGCTGCCAATTTAAAAACAACCATGAGGAAAGGCTTATCGTGGAGA10.6172839506172839No Hit
CTAAAAAACATGCTACTGACCAACCAATTGGGTTCATTGGCAAACCAAAA10.6172839506172839No Hit
GTGTGGGGACCAGCCAGGCGGCAGAAGCACAGGTCGGGGTGGCAGTTTTT10.6172839506172839No Hit
GCTAGGATGGGAATATGGGCTGGTTGCAGAGGGAAGGGCACTGAGAGGTG10.6172839506172839No Hit
GGCCCGCGGTTCCTGCCAGGGCCGGCTCTCCTGCACCTCTTCTTCACTGA10.6172839506172839No Hit
GCCGGTGGGGAATCTATGGTTAAGGTAGAAAGGGATATATCTTCTGATTT10.6172839506172839No Hit
GATGACCTCCAAGGGCCCTTCCATGTGGGTACCTCCATCCCCAGGGGCAG10.6172839506172839No Hit
TTCTAGGGGCACTTGAGAGGAGTATATTCCATTTGCTGCTTTTGGATGGA10.6172839506172839No Hit
GAGGTGAACATATCAGGTCTTGTAATAGTGGCTAAAAGGTACTCCACTTG10.6172839506172839No Hit
GCACTTAATTTAACATATATGGTTTCTTTTTCTTATATAATATTTTTTTT10.6172839506172839No Hit
GGGTGAGGCTGGAGGGAAGCAGGCAGGGAGCGGGAGTGGTAGGTGAGGAC10.6172839506172839No Hit
GATCTGAGGAGCCCATTTAGCAAACTCAAGGCTGAGACGTGAAAATCAGA10.6172839506172839No Hit
GCCCATGGGCTGGCATTACCACAGCATTGTTGACGGATTCACCGTCCTTA10.6172839506172839No Hit
ATATTTCATTTATTTTAGAGCGAGAGAGAGAGTATGCACAATTGGGGAAA10.6172839506172839No Hit
GCTTCAGAGTCTTCAAGGTATTCTAAATATTTAAGTTTGCCTTCATCTAT10.6172839506172839No Hit
ATAATGTTCTCAAATAAAATGGCGAGGTTAGGAAAAGGATACATGTTCGA10.6172839506172839No Hit
GCTCATAAGAATGTACTATGAACAATTACACCCCAACAAGCTGGATAATC10.6172839506172839No Hit
GAAGTAATGGCAGGTCATCCGTCCATCCAGATCAGGAGAGAGCCCATACA10.6172839506172839No Hit
TGCCTACTTCCAAATCTAAAATGCCCTTCCTGATATTTGCTCCTAATCAA10.6172839506172839No Hit
CTTCTCATGCTTGCAATGTGCTCAATTGGGGCAACATCTTCCCCTATCTC10.6172839506172839No Hit
GAGTGGTTTCAGTTTGCCTTTCGCTCCTTGGCTCTGAGCAAGTATTCCTA10.6172839506172839No Hit
GTCTGAAACCTTCCTTCCTTGCCGTCACTCCTGGAGCCAGGAACAGGAAG10.6172839506172839No Hit
GGCCAGCTCTGTCCAGGACACTCATCTGCCTGTTCCAGGTACTTCCTGCT10.6172839506172839No Hit
TCTTCATCGGGGGCCGGGGCTCCCCTGGAGACAGGCAGCTCTGGAGGGGA10.6172839506172839No Hit
ATACACAAATAACAGGGGAAAGAGCATTCTTTTCGTGTGGGGCATACATC10.6172839506172839No Hit
GTCCTGAGCCTCTGGGCCCAAGGAGACAGCAGCTCCTTGCTCACCTGTCC10.6172839506172839No Hit
ACCCGGGTGCCGCTGATGACAAAAGTTTCAAACTTGGGTTTAACATGATG10.6172839506172839No Hit
GGCACATTGATAAGGGCTGCACGAAGGCCAGCCATGGCCACTGCTGGCTG10.6172839506172839No Hit
ATTACACCCCAACAAGCTGGATAATCCAAAAGAAAAGGGTACATTTCCAG10.6172839506172839No Hit
TATTTGTGTATTGGGCGTCTTGAACAGGGTAAAAACCGCTCTTTTGAGTC10.6172839506172839No Hit
GCTTTTAAGATGTTCAAGAGAGACTGAAAACATTTACTGGGAGAAGAGTC10.6172839506172839No Hit
GGCTCTGAGAGCAAGTGTGAGAAATGAAGGCTGTGATCCGTGGTGTGCTT10.6172839506172839No Hit
ATCTCAGGGTCCTGGGATCGAGTCCCGCTTTGGGCTCTCTGCTCATCAGG10.6172839506172839No Hit
CTCCACAGAACTGGGGGCACAGTCTGAAAACAACTGAATTGTGGGATCTC10.6172839506172839No Hit
TTCCTTGACCTGTATCCCTGTGATCTGATGAAATGCTTATAGATTTTTCT10.6172839506172839No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA10.6172839506172839No Hit
CCCCTGCATTCCTGGGATAAATTCCACTTTGGTTAGTCATGGGTATAATA10.6172839506172839No Hit
GGGGCAGTGTCACCATCCTGAGATCCTGGCCTGAGCCAAAACCAAGGTGT10.6172839506172839No Hit
GTCCAGATGGTTTCAGTGGATCTCTACCTAATCATGTCATTTTGTTTCAT10.6172839506172839No Hit
GGCACAAGATTTGGCCAGGATGGGCTCCATTGGTGCAGTGGGAGCGCCCA10.6172839506172839No Hit
CTATTCCCATGTCATGGGGTCGGTGAGCATGACCCAGGACTAACACACGG10.6172839506172839No Hit
GTAGAATATTGAGCTCCTTGACAGAACATGCCTCTAGAAGGAAAAGAAAT10.6172839506172839No Hit
CCCCAAGGTGAGCCAGAGCATGGAAGGCACTGGGTTTGCTCTGAGCCTTT10.6172839506172839No Hit
TTGCAATAATGTTGTAAAGTTGAAATGGAATGATGCTTCAAACTCTCTTA10.6172839506172839No Hit
GTCCCTAGCGGTCACCTCCAATGACCCAAACCACCCACGGGGCCCCTGTC10.6172839506172839No Hit
CACGCTAACAGAAACCAATGTTCCTGTGACACATGCCAAAGAATTGCTCC10.6172839506172839No Hit
GAATGTACTATGAACAATTACACCCCAACAAGCTGGATAATCCAAAAGAA10.6172839506172839No Hit
CAAGAATGCAGAGACTCATTTCCAACCCCCATAAAATCAAAAATTTGGAA10.6172839506172839No Hit
GCACAAGAGGGGAGGGCACGGGGACAGGCCTCCTGTCCTGGGAACACCCA10.6172839506172839No Hit
GTCCTCGAGACAAGCCTGATCCTGGCTTTGGTTGGAAAGAAATGCAGGTT10.6172839506172839No Hit
CTGCCTGCCTCGGCCTCCCGCCGGCCAGGGGAGAGCTTGCAGTTTCCGGA10.6172839506172839No Hit
GGGAGAACTAGGCCTGTTTCCCGGGAACTGTCAGTGCAGAATTAAGTGTG10.6172839506172839No Hit
GCCCCCATACCAGTCTTGCTGTGCAGGGTTCTGTTTGGGAGGCTGAGAAT10.6172839506172839No Hit
GCCCAACACTGGCCAGAGCTCCCATGAGTGGAGTGAAAACACCCCGAGCG10.6172839506172839No Hit
GCTTTACTTTCAGAAGCTTCTAGTCCTTACCCAGGCCCTTCGTAGATCCT10.6172839506172839No Hit
ATCTTGGGGGACTGGAGATGCATTAAATCCTCACACTTAATGGATGATCT10.6172839506172839No Hit
GTGTAGGGGGAGGCCCTTTGATTCTGCCGGGCTGATGAATGTCTTCTTGG10.6172839506172839No Hit
TGTTAAGAGTGGCTCATAGGAGTCAGAGAAGCAATTTGCTTAACCACATC10.6172839506172839No Hit
GCTTTCAGAATGCCTGTCATCAGAGGGGGGGCTTACAAACAGGCAGCAAA10.6172839506172839No Hit
CCAGTGCACAGCGCAGTGGCTGGTAAGTAATGGGGTACGCTGTGTTCATA10.6172839506172839No Hit
ATGTTAGAGTAGTCACGGCGGTTCTGATTGCAGGGGGAGGGACCCAATGT10.6172839506172839No Hit
ATGGAGAATGGAGGGCTGGGAGGGAGGGAGACATGCAGTGGTCTGGGCAG10.6172839506172839No Hit
CTATTATGTTATTTCTCTGTTTCCCAAAAAGACTCTAGAGGTGAAATACA10.6172839506172839No Hit
CCACTATGGACTCTTCCTTTGAAGGTAGGAGACAAGCTCAAGGAGAGACT10.6172839506172839No Hit
ATAAAGTACAGATAAATGGTTTGGATTCATCAGTCTCCTCTCCACAGAGT10.6172839506172839No Hit
CTTCTCCCTCTGGCCTCAGTTTCCCTAATGACAGAGTGAGGGTATAGCAC10.6172839506172839No Hit
GGAAGAAGGGGCCGACCACCAGGGCAGGGGCACCAGGGACTCTGCCATCA10.6172839506172839No Hit
CCATGGAGCAATTAGATTCCCATTGGATCGTACTCGCAATGTTTGGCCTG10.6172839506172839No Hit
CTACAGGACAGCAAGCAAACTGGCTGCACAGGTCAGTCTAAGGACGTCTT10.6172839506172839No Hit
GTCTAGAAATACAGCCCTGAAATCAAATCTTTGTGGGGCCGTTTGGGCTA10.6172839506172839No Hit
CTCTCCCTCTGACCCTTCCTTCTCTAGCATATGCTTGTGCACTCTCTTTC10.6172839506172839No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG10.6172839506172839No Hit
CAACACGGCACTTCTTTCAAATACATGCTTTGGCCACACCTCTCTACACA10.6172839506172839No Hit
GAGGTGGGGGGATTGCACTCTGGGAAAGATCCATAGAATCCAGTTCCATT10.6172839506172839No Hit
GTCTCGCTAGGGGTGTGTGTGTGGGTGTGAGAGAGAGAGAGAGAGAGAGT10.6172839506172839No Hit
TCCCAGGACCCCAGGATCATGACCCAAGCTGAAGGCGGACACTTGACTGA10.6172839506172839No Hit
GGTTAGAGAATCAATGAGCTGAAGGGAATTCATACCTGGTAGTCTTGTTT10.6172839506172839No Hit
GAATAAGGTAGAGTCAGAATCTAGCTTTCTTTCTCATCTCCCTATTTGCC10.6172839506172839No Hit
CTCGGTCTCCCTCCCTCGGGGGACTCCACAGAACTGGGGGCACAGTCTGA10.6172839506172839No Hit
GTTCAAGATAAGGCTTTTTTCCCCCCTCCTTTTTGCTTTTCTGGAAGCAG10.6172839506172839No Hit
GCACATTAGTTTGATTTGTGGATGTAGAACTGACGTTGCATCCCTGAAAT10.6172839506172839No Hit
CAAGCAGGCACTTGGGCAAAACCGGCTGCCACAGACACCATTTAGATCTG10.6172839506172839No Hit
ACCTTGGACCAGCAACAGCCCAGATGGCTCTTCAGCTGTTCATTAAAGAC10.6172839506172839No Hit
TTCTCCACACCTGTCTTCATTCTCTTCTGGTTTGGGTCTCTTCTCGTCCT10.6172839506172839No Hit
TCAGAAATGAGAGAGATGTTCCCTGAAACCATTGATATGCAAAGGCTCTT10.6172839506172839No Hit
CTATTAACTCTTACATATTTGGGGCAGGTTCCAAATGCATATTTACTGAT10.6172839506172839No Hit
GGCCGGGGACGGCCGTATGCGACAGTGCAGAACGCAGTTTCGGTCGGCTG10.6172839506172839No Hit
GCCTTGGAGCTTGGAATGAGCAAGTATCAAGAGCAAATGTTCAGACAGTT10.6172839506172839No Hit
GAGTTCCAGGGGCAAACACACTACACACATCCACTGTGTTTTAAAGCTCA10.6172839506172839No Hit
GTGGTCCTCAGACCAGCTGCTATATCACCGTGAATCTTGTTAGAAACGCA10.6172839506172839No Hit
TCTTTGCCCTCCGCCAGACACCAGGGTCAGACACCAGAAGGTTCTCCTCC10.6172839506172839No Hit
TCACACTCCCACTTCTGCCATGCAGGGGTCAAGGTCCCTCGCTGGGAAGC10.6172839506172839No Hit
GGATAGAGGTTGGGGGAGCAGAACTAGGGTCATGTCAGTGGAGTTGGATC10.6172839506172839No Hit
CCAGGGGGGCCGTGGGAGAGACCTGTCAAAGCATCTCTTGTCGGCCTGTA10.6172839506172839No Hit
TGCTAGGGCAGCTATGGCCCTTGGAGGTACCTTGCTGATTGGAGGCTATT10.6172839506172839No Hit
CGCGCACACATACACACATGTGCACACACATGCACACCGTGCTCAGGAGA10.6172839506172839No Hit
TCCTCTACTTTATATAGAATGACCGGTCCGCAGACCGACTGCCAGTTTGA10.6172839506172839No Hit
GACTAGTCCTGGCCAACCTCCTTCTATGAATCCAGCTATGGCTCCAAATA10.6172839506172839No Hit
CATGTGAATTGACCGATTCGAGCTGGATAGAACTCGATGAGATAGGGGAA10.6172839506172839No Hit
GCCTGGGCCTGGGTCTTGGTCGCCTTGTCAGCTGGCCTTGGGCCACTGAG10.6172839506172839No Hit
ACTCCGGGGAGAGTCCCTGCTTTCTGTGTGTGCATGCACACGCGCTCGCG10.6172839506172839No Hit
CTCCCATAGAGGATTCTTTAATTTAACAGGGCTGATATTGTTTCTGGGTT10.6172839506172839No Hit
GCCAAGAAGGAGGCGGTGGCAACAGTGTGTGCTATGGCATGTTCCTCGGG10.6172839506172839No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers