FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1911-d12-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1911-d12-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992078
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG3350.030.3028582
CGTAAGA1255.149482E-429.0041642
TATACTG3900.027.8886185
AGCGAAA3503.6379788E-1226.937871
GTTACGT1358.0663816E-426.8611241
CGAAAGC4101.8189894E-1224.7596513
TTATACG1500.001493015924.1701374
TAATAGT3004.548565E-721.7531244
TATAGTG4701.0913936E-1121.5988455
GTTATGT6950.020.8705141
TATACTA2104.309921E-420.717265
ATATAGT3301.1363954E-619.7755664
TTACGTG1850.005042659619.5974082
ATATATC5555.456968E-1219.5974063
GATATAT4152.4800102E-819.2235031
TATAATA5305.638867E-1119.1536922
CTGATCG1900.00588811419.0788029
GTACCTA6101.8189894E-1219.0191256
TTATACT4253.2663593E-818.76744
GTATAGC2359.1786875E-418.517031
Produced by FastQC (version 0.11.5)