FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1911-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1911-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575352
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG1200.060.4165659
GCGAAAG5950.030.4630992
CGAAAGC6250.029.0008683
AGCGAAA6650.026.1678581
CGACTCG2259.113228E-725.77773145
GTACGGG3757.768904E-921.2673056
GTATAAA8150.020.4620361
TATAATA12350.019.9601122
GTATTAA14800.019.5964261
GTACTCG1950.006841077518.5903023
TATTATA7900.018.354982
TAGTACT5651.4370016E-1017.9651414
CGTAGGA5251.0404619E-917.9529192
GTATATA8550.017.8086831
CACGACG2450.001217361317.755077
GTATAAT5751.8371793E-1017.6538241
TATAGGA5501.9317667E-917.1368772
GTATATG8900.017.1083411
TATTAAT12150.016.7083192
GGTATAA5252.017623E-816.5729771
Produced by FastQC (version 0.11.5)