FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1911-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1911-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1435682
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTATGGCTGTTCGCCATTTAAAGTGGTACGCGAGCTGGGTTTAGAACG19970.13909765533035867No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT16950.11806235642711967No Hit
GTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACATCTTCGGCGTTGT16120.11228113189411025No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG800.002035918636.2535974
TGATCCG6400.032.8513879
AGCGAAA7050.030.8584251
GTATATA6900.028.3763351
GTCATAG7850.026.7860982
GCATCGA4750.025.947231145
AGTGATC8900.025.2526767
CGAAAGC9400.023.9119453
GCGAAAG9300.023.3894162
GTGATCC10600.021.2027198
CATAGTG10150.020.7163414
ACCGATC2455.1162035E-520.7141768
CGTAGTA2155.0542294E-420.2345642
CTACCGA1800.00430549820.1408866
GTATTAA11650.019.9189151
GGTCATA12400.019.2989581
GTATAAG5657.2759576E-1219.252381
GTGTTAA7650.018.9587691
TTCCGAC9950.018.9466044
GTATAGG4603.92356E-918.9175571
Produced by FastQC (version 0.11.5)