FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1911-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1911-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800156
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG1950.044.615219
AGCGAAA3300.030.7651441
GTATTAT6500.027.8914781
GCGAAAG3750.027.0682532
CGAAAGC4051.8189894E-1225.06323
CTGCGTA2351.3299286E-624.6807549
GTATATA4201.8189894E-1224.1726151
GATGCGC1801.5199557E-424.166578
GTCCTAT9300.023.392851
CTACTGA6200.023.3884686
ATTAACG1952.6130737E-422.3093
GTACCTA2353.7063113E-521.597016
CATAGTG4058.039933E-1021.4827424
GAATAGT3851.0473741E-820.7193831
AGAACCG2455.110057E-520.71555
AATACGA1750.003656858820.71555
TAATACT5301.8189894E-1220.5200714
TCCTATG10250.020.5133972
TCCGACT3551.01344995E-720.4237355
GTGCTAA6450.020.2375371
Produced by FastQC (version 0.11.5)