FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1911-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1911-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences712179
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC11030.15487679361508833No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACCG553.2183944E-452.72725
GCGTTAC450.00963004348.3434451
GCGAAAG2300.037.826032
TATAGCG800.002036236236.249952
TATACTG2850.035.6139835
CGAAAGC2551.8189894E-1234.1175963
GCGGTAT900.00362756132.2221766
TAAGGCG900.00362756132.2221765
GTCGGTA2558.913048E-1131.274464145
ACCGTTC1652.3851826E-630.757538
GGTACTG2859.094947E-1230.5262725
TCGCCAT3350.030.298464145
GTATAGG5400.029.5432191
GGATAGC3450.029.4202465
CGTTATA1050.00769912127.6248251
TTATACT3453.6379788E-1227.31884
GTACTGA4100.026.5243536
TATTAGT4900.025.1530232
AGCGAAA3551.546141E-1024.512171
GTATATA2451.9073213E-623.6784231
Produced by FastQC (version 0.11.5)