FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n01_1910_rep2_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1910_rep2_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1662
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATCTACTGAGGGGAGAAGAGCCAACGAAAGATTCAACCTACCGCTGC20.12033694344163659No Hit
GTCCACATTAGTTATGGGAATCCAGCAAGAGAACAGACAAACCAGAGAAG20.12033694344163659No Hit
TATATGAACAAACCTAAGAGAAGCAATCAGCACCAATACCATAGGGAACT20.12033694344163659No Hit
GGGGCGAGGGGCAAGCACAGAGCCATTTTTCAGAGATTCAAAATGAGGCC20.12033694344163659No Hit
CAGTTTTGTCACCTGTAAAATGGGTATAATAATAATGCTGATCGCCTAGG20.12033694344163659No Hit
TTTATGAGCTGTGTGCCCCATGGCAACTGGCTCAGCTTTCTGAGCCTCAG20.12033694344163659No Hit
GTTACTAGCCTGCTGGACCTCTGGAGTTAGAGGCCCTGTCTTGGCACAAG20.12033694344163659No Hit
AGGTAGAGCTGGTGAATGGCCGTGGGCACCTTGGGGGGCATCTTCCTCAG20.12033694344163659No Hit
GTGCAGAAACATTTTATCTTGATGAAGTCCTAATAGTTCATTTTTGCTTT20.12033694344163659No Hit
GGTCTGAACACCTTTGTAAGCCCTACAGTTCCAGAGCTTGCTACTGCTAA20.12033694344163659No Hit
GTCTGGCTCCCGTAGCGGCGCTGGCAGGGGCCTGAGCCCCGCAGCCCTCG20.12033694344163659No Hit
GTTACAAGCTGTAATCATTTTACTGATTTCTATGTTCATTGGCTTTTGGT20.12033694344163659No Hit
CTACACAGCAGATTTAAAAATACAGAGTGCTAAGGTTGGCACAGGGGCGA20.12033694344163659No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT20.12033694344163659No Hit
TCAGTTGCCTTGGCTGTTTCTACCCTACAGCCACAGAGCCTGACTCCATC20.12033694344163659No Hit
GAATTGGGGTTAGGTTCAGAGTGGAAGGTGAGCAGGCAATCAGGCTTACT20.12033694344163659No Hit
ATCCAAACCTCTCACCGTGGTGTTTGAGGCCCTCTGAGGCGCCCCCCCAG20.12033694344163659No Hit
GTCTTGGCACAAGAGTTGGCCAGGATGGGCTCCAGGGGGGCCGTGGGAGA20.12033694344163659No Hit
CCCCACTTACAGCGTGAGAAAATCATCCTGTTCCTAGAAGAGGGATGTTA20.12033694344163659No Hit
TTTCAAATCATTTTTAACAACACGGCACTTCTTTCAAATACATGCTTTGG20.12033694344163659No Hit
GAATAGGGGAGACCCGCCGTCAGGAAAGTCACTGCTGCGGCATCCGGGCA20.12033694344163659No Hit
CTACTAAGCTGTAAGCTTCATGAAAACAGGAACCAGATTTTGTATCCTGA20.12033694344163659No Hit
CAGCTGACATTGTCCTTGGCGAAACACCAGAATATGAGCTCGGGGTTAAG20.12033694344163659No Hit
CTTTAATACCTCACACTTCTCTCAATGATCCCCCATCCAGACAGAAGATC20.12033694344163659No Hit
CAGCCACAGAGCCTGACTCCATCCAACCATCTTTTCCTGGGTTGGTAGCC20.12033694344163659No Hit
AGTTGGAACTAGGACCTCGGATGCCAGGCTTCTGCCCTGGTAACATACCC20.12033694344163659No Hit
GCATGGAGCAACTAAATATGTAGAGCATATATGAACAAACCTAAGAGAAG20.12033694344163659No Hit
ATCAAATGCTTTTTCTGCATCTGTTGACATGGTCCTATGATGTTGATCCT20.12033694344163659No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT20.12033694344163659No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)