FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1910-rep2-d12-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1910-rep2-d12-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1046309
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAT3750.036.741931
AGCGAAA4450.035.8510861
GCGAAAG4650.034.3041882
AGACGGT4200.032.797455145
CGAAAGC4950.032.2251433
CTATATA4600.031.5245952
ATATACC5200.029.28154
TTACGGT1000.00607189429.0026285
TATATAC6700.028.1368773
GTATATA4900.028.1188261
TATACTG4550.027.0903685
GTGATCG2507.831841E-826.0998739
CTATAGT3451.07320375E-1025.2232931
TACACGT1450.001225833125.0022665
TATACCA16100.022.0672195
CTGATCG3655.686161E-921.8492079
TACCATG6800.021.3234237
ACGAAAG4201.2678356E-920.714184145
TCTATAC13000.020.6364863
GAATAAC4601.7644197E-1020.4939251
Produced by FastQC (version 0.11.5)