FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1910-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1910-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336740
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC15530.11617816478896419No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG14590.10914613163367595No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTA400.006051423554.374786145
TATACTG6200.031.5736395
GTTATGT8650.029.3461251
CTAGTAC1255.1537453E-429.0009733
GTTACGT1804.687994E-628.2048851
GTAATAT4150.027.962161
CAACCCG3650.027.808111145
GCGAAAG4450.026.068292
AGCGAAA4500.025.7873251
TAAGGTA6550.025.4588684
GTATAAG2301.1000247E-625.2267281
CCGACTA2901.1910743E-825.0008374
TAGTGCA7550.024.9667939
GTATTAT13450.024.804721
TATATAG4750.024.4218715
TAGCATG15750.024.3976422
CAGTGCA8350.024.3121932
TAGTCGG2401.598808E-624.1665738
CTACTGA13250.024.0762796
CGAAGGT1851.8306989E-423.5143034
Produced by FastQC (version 0.11.5)