FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1910-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1910-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823045
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG9270.1126305366049244No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCG657.335824E-444.6153835
GTTACGT1105.263164E-639.5454561
CATCGTT1209.561794E-636.252
GTTGACA7150.029.4055941
TTACGTG1606.8266905E-527.18752
AGCCGAC1100.00967060726.3636382
GTATTAT6700.025.9701481
GTTATGT3357.4578566E-1125.9701481
ACATGGT8750.024.8571435
TAGCATG10300.024.6359212
ATATCAC3352.1082087E-923.805973
GACTTAA3052.0336302E-823.7704931
TAGTACC4353.6379788E-1223.3333329
CATTACC6250.023.23
GCATGTT11300.022.4557514
CTATAGG1952.6141998E-422.307691
GTAGCAT15600.022.307691
TATACTG4607.2759576E-1222.0652185
ATAATAC8250.021.9696963
ATACAGC7050.021.5957436
Produced by FastQC (version 0.11.5)