FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n01_1910-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n01_1910-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences691178
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG10120.14641669729071238No Hit
ACACCTAACAGACCAAGCCACACAGGCAGCCCTTAGAGCCAACTTTAAAA8270.11965079907057226No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT7580.10966784243711461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1100.072.515091
TTACGTG1100.072.515092
TAGCTCG350.00356309962.155795
TACGGGG400.006050314854.3745087
GTTATGT4450.047.2570231
ATACCGC750.001481553338.6747135
ACGTGTT2109.094947E-1237.9840934
CCGACTA2500.037.7078444
CGTGTTG2151.2732926E-1137.1007425
GTATATC1458.679108E-735.0072861
GTTGACA5300.034.205231
TATACTG3000.033.8403745
GTAACGG1102.4324015E-432.9614031
GCGAAAG2455.4569682E-1132.5577931
TACGTGT2455.4569682E-1132.5577933
CGCATAC900.003623767332.2289282
TTATGTG7300.030.7940792
GGTTATA3800.030.5326671
GGACGGC1952.600591E-729.7497772
CTACTGA8300.029.7049756
Produced by FastQC (version 0.11.5)