FastQCFastQC Report
Thu 6 Apr 2023
HK77KDMXY_n01_NYULF_0764.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77KDMXY_n01_NYULF_0764.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41841274
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGTTT466730.111547750673175TruSeq Adapter, Index 11 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGTAT466480.11148800105847637TruSeq Adapter, Index 11 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)