FastQCFastQC Report
Mon 10 Apr 2023
HK77KDMXY_n01_NYULF_0764_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77KDMXY_n01_NYULF_0764_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43250585
Sequences flagged as poor quality0
Sequence length151
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGTTT474900.1098019830252007TruSeq Adapter, Index 11 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTACCAATCTCGTAT474450.10969793818973778TruSeq Adapter, Index 11 (97% over 39bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)