FastQCFastQC Report
Thu 6 Apr 2023
HK77KDMXY_n01_NYULF_0218.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77KDMXY_n01_NYULF_0218.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35710759
Sequences flagged as poor quality0
Sequence length151
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTTGAATCTCGGTT594160.16638122981368164TruSeq Adapter, Index 5 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTTGAATCTCGTTT473230.1325174858366914TruSeq Adapter, Index 5 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTTGAATCTCGTAT390520.10935639872566136TruSeq Adapter, Index 5 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)