FastQCFastQC Report
Mon 10 Apr 2023
HK77KDMXY_n01_NYULF_0218_a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77KDMXY_n01_NYULF_0218_a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36324376
Sequences flagged as poor quality0
Sequence length151
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTTGAATCTCGGTT600470.1653077261396039TruSeq Adapter, Index 5 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTTGAATCTCGTTT478250.13166089900622105TruSeq Adapter, Index 5 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTTGAATCTCGTAT393860.10842856598555195TruSeq Adapter, Index 5 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)