FastQCFastQC Report
Thu 6 Apr 2023
HK77KDMXY_n01_NYULF_0139.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77KDMXY_n01_NYULF_0139.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46503115
Sequences flagged as poor quality0
Sequence length151
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGTCATATCTCGTAT2551140.5485955080643522TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGTCATATCGCGTAT797230.17143582747091243TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)