FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5684693
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA2600.044.547876
CGGAGAT4800.042.2254379
CACTCTT8600.038.6886752
TCGGAGA4400.037.8377158
CTCGGAG3950.034.8206527
TCTCGGG6700.029.457567145
TCCCTAC4850.028.3575869
GGCTCGG3501.3278623E-1024.8175625
TTCCCTA6050.022.732548
TCCGATT3254.098729E-822.2554991
TATACTG6050.021.53595
TATCGGG2053.4711722E-421.394655145
AGCGGCC4853.237801E-1019.593388145
GTCGCCC6350.019.56965145
CCGATTT3802.1346932E-719.0344732
GGGCTCG3653.048348E-617.840544
CGCATTT3703.147663E-617.780693145
CGGTGGG12800.017.70352145
CCCGTAT2050.00925553217.6415521
TAGATCC6454.5474735E-1116.999628145
Produced by FastQC (version 0.11.5)