FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n02_MG_positive-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n02_MG_positive-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8346682
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAG33900.039.3463367
GACTCCC33100.037.66922145
TCTCGGG46000.034.039417145
CGGAGAT65200.027.4623769
TCGGAGA48600.027.1469678
GCTCGGA39600.026.9095086
GACCCCC34550.020.561945145
GTATAGA33500.017.5280761
CGGTGGG94600.017.011713145
CTACACT22600.017.0006944
TACACCG52350.016.8944855
CTAGACT10400.016.7292754
CGTGTAA52200.016.525806145
TAGGACT19200.016.2355294
TGGGCTC26400.016.2012043
TCTACAC32950.016.060783
CCTACAC32350.015.910483
TACACAG30100.015.8956845
TAGACAG25000.015.6586935
TATACTG39150.015.5542575
Produced by FastQC (version 0.11.5)