FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n02_MG_neg.8.7-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n02_MG_neg.8.7-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13402
Sequences flagged as poor quality0
Sequence length151
%GC54

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGGGCTCGGAGATGTGTATAAGCTGTCTCTTATACACATCTGACGCTG590.4402328010744665No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAT100.007013535145.06
TCCTATC100.007013535145.07
GTGGGCT151.20330944E-4144.999982
GCTCGGA256.23318E-6116.06
TCGGAGA301.5422986E-596.666668
GGGCTCG259.1845E-487.04
TGGGCTC259.1845E-487.03
CTCGGAG353.3147826E-582.857147
CGGAGAT406.426404E-572.59
CGTGGGC300.00189418772.499991
GGCTCGG350.00349021962.1428575
AAAAAAA7203.6750498E-75.0347223135-139
Produced by FastQC (version 0.11.5)