FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n02_MG_8724.tr6-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n02_MG_8724.tr6-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8998923
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT56000.080.9028859
CTCGGAG49050.075.37047
TCGGAGA48500.074.581468
GCTCGGA35950.074.201696
GGCTCGG32400.052.5783585
TCTCGGG70750.047.233112145
GGGCTCG27300.044.8753854
ACTCGGA19550.039.673626
CGACGAA68750.037.641556145
CGGTGGG96850.034.953392145
TGACGCC39200.034.76513145
TGCTCGG29850.025.2564965
TCTCGGA22150.025.1989146
CGTGGGC35750.025.1437341
CACGGAG29950.024.4452347
TCGCCGG142950.023.985514145
CGTATCC82250.022.561924145
ACGGAGA33400.022.354398
ACGATAA60850.021.562048145
GCACGGA23250.021.200736
Produced by FastQC (version 0.11.5)