FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n02_MG_8724.tr3-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n02_MG_8724.tr3-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9880224
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT40550.079.553129
GCTCGGA26350.077.579256
TCGGAGA35450.075.047418
CTCGGAG40650.067.230227
GGCTCGG23200.052.1828235
TCTCGGG67050.051.139046145
GACGAAA81300.044.67221145
TGACGCC29800.041.84104145
GGGCTCG19900.038.6144374
CGGTGGG91800.036.56189145
ACTCGGA19750.027.5277846
TGCTCGG20050.024.9478875
TCGCCGG125850.024.13521145
TAGCTCG11600.022.497874
CGTGGGC24500.022.1917741
TGGCTCG18200.021.110644
AGCTCGG23450.020.4032885
CGTATCC53100.019.385817145
CGCGGAG8800.018.9464937
CACGGAG25000.018.8476437
Produced by FastQC (version 0.11.5)