FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n02_MG_8337.tr2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n02_MG_8337.tr2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10910359
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT49450.092.501659
TCGGAGA38200.087.672438
GCTCGGA31450.087.587756
CTCGGAG45350.077.525917
GGCTCGG31500.055.0036665
GGGCTCG25350.048.043614
TGACGCC33700.045.387573145
TCTCGGG86350.045.24919145
GACTCCC75400.041.725616145
TAGCTCG11550.036.4041024
ACGTGTT42050.034.650894145
GCCGACC29750.034.357105145
TGCTCGG21600.033.5622145
GCACGGA17350.033.4249656
CGCGGAG10550.032.294447
CGGTGGG122950.030.069492145
CACGGAG28850.029.9008547
CGTGGGC31300.029.8777371
ACTCGGA18000.029.8016056
GTCGCCC54700.029.685553145
Produced by FastQC (version 0.11.5)