FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n02_MG_7784.tr2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n02_MG_7784.tr2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9396787
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT40650.042.7998929
TCTCGGG62000.040.33847145
GCTCGGA26850.038.068396
TCGGAGA38400.035.491038
CTCGGAG41900.032.3532037
GACGAAA79050.029.070293145
CGGTGGG87750.026.353334145
TGACGCC45450.022.32987145
TCGCCGG134350.021.529167145
GGCTCGG29700.020.2595525
TACACCG24600.018.56585
GGGCTCG28800.016.8650724
TACACTG58200.016.5667325
CTACACT47250.015.8030634
CGTATCC56600.015.369396145
TATACTG72750.015.34602455
GTATTAG59300.015.036831
CTATACT52400.014.5265974
AGTGTAA122050.014.492525145
ACTCGGA20050.014.4622326
Produced by FastQC (version 0.11.5)