FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n02_MG_7379.tr2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n02_MG_7379.tr2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9955085
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT46300.062.6274539
TCGGAGA35300.057.9108778
GCTCGGA31700.054.653216
ACGATCC29000.048.744373145
TGACGCC40650.042.799488145
CTCGGAG50050.042.002097
TTACGCC24600.028.584099145
GCCGACC30750.028.525162145
GGCTCGG38500.027.3025955
TACGCGG14050.025.797735145
CGACGAA184650.025.753864145
GGGCTCG30100.025.2882884
GCGTGTT88500.022.279917145
TCTTACC68950.019.66053145
ACGAACC22250.019.222502145
ACGGAGA32000.018.8024238
CGCGGAG18050.018.4738587
GCGCGGA18200.018.3216026
GCACGGA22950.017.3722886
CACGGAG40900.016.3057797
Produced by FastQC (version 0.11.5)