FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n02_MG_341.tr1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n02_MG_341.tr1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGTAGATGTTGAAATAATCCTCTTTTTATGCCCCTCAGCACGTTCAA11.6129032258064515No Hit
GCATCTAGGAGGTTAAGATGGGAGGATCTTCTTTTTTTTTTTTTGAATCG11.6129032258064515No Hit
CGACAAACGAACTTAAAAAATTGAGTAAGAACAGCAAATGATCAAATGAT11.6129032258064515No Hit
ACCGGGAACTGTTCAAAATCGTTCTACCTTAACTGGACAATAGACCCTAC11.6129032258064515No Hit
CGAATACGTCGCATATCCACCGCACCGGCAGCGTTCCTACCCCTACGCAA11.6129032258064515No Hit
CACCTACAGAGATGAAAATGCACACCTGCTAATAACGTAAACGCCGTAGC11.6129032258064515No Hit
GAAGTAAAGTTCATGGCCTTTGTTACACCCATTTTCGCTTTTTTTTTTTT11.6129032258064515No Hit
GGAGATTTAGACTGCTTTTTGGTTTATGAAGTTTGGCTTAAGACTTTTCA11.6129032258064515No Hit
GTGTTAGGCTTTTTTTTTTTTTTTTTTTCTTTAAAACAGCATCTCCTGTC11.6129032258064515No Hit
CATTCAGATAGCTGGACTCCACCAGTAGAGCTTCCATTCTCTGGGTCTTG11.6129032258064515No Hit
TATGCTTAGAAAAGAGCGGTTATAACAACTCAGCCCACAGGAGACCCAGA11.6129032258064515No Hit
ATAAAATATGCTTGAGTAATGTATATGTAAATATGTATACCTACGTATAT11.6129032258064515No Hit
CTATAATGTCATGAGAATTGTGTTGGCTAGGAGACGAATAGCGCTGTCTC11.6129032258064515No Hit
GAATTTAGAGCTGGAACCAAGAGACTCAAGATCAAACACTAGCTGTGCCA11.6129032258064515No Hit
TCCTTTCTCTTTCTTTTTTTTTTTTTTTTTACTTTTAGCTACCCCTCAAT11.6129032258064515No Hit
GAACTGAACTGTGTCCCTCCCAAATACATACTTTGAAGTCCTAACCCCCA11.6129032258064515No Hit
CCCTCGTTGTAGCGACGGCCCGATAGGACACGGCCGGTGAACTCATCGAC11.6129032258064515No Hit
GTACAGTTCAAGTTTCTTCACTTTTACTTAAACATAATATATAATGGATA11.6129032258064515No Hit
GGGCAGGAGTACTTTAAATCAGGTATGCAGGGGGATGGGCAAGCCCTTAC11.6129032258064515No Hit
GTACTGGAAGTCCTAGCCCGAGCAACCAGGCCAGAGAAATACATAAAAGG11.6129032258064515No Hit
TTGTCGGACAGGTTGTCGAGCTCGTTCACGTCAGCGAAGTGGTCGGCGAC11.6129032258064515No Hit
GGATCATATCATGCATGCTGTCTTTTTTTTTTTTTTCCTTTTCAGATGTC11.6129032258064515No Hit
ATCCAGATCTTTTTATACTCAGATTATGGCTTTTCTACACTTTATGTTGT11.6129032258064515No Hit
CTATCATCTACACTACGTCCCATCACAAATTACCCACTCCTATAATTCTC11.6129032258064515No Hit
AAACTGAACTGCCCAAGACTTAGCAAAAAACAAAGCACAGCTGCTTCTCT11.6129032258064515No Hit
GGGTTTCACCATCTTAGCCAGGCTGGTCTCGAACTCCCGACCTCAGGTGA11.6129032258064515No Hit
CAATTATTCCTCAATTAAGCTTAAAACATAATATGAGCTCAGGATCTGTC11.6129032258064515No Hit
TAAAACGTATTATTATTATTTTGGATAGGAGGAAGGATTAACAGACGTGA11.6129032258064515No Hit
ATATAATGTACCATCCCTCTTTGTCTCTTTTAACTGCTGTTGCTTTAAAA11.6129032258064515No Hit
ATTTTCCACAAGCATACCTGAAAGCTAACATGAACAACAGACATTTTAAA11.6129032258064515No Hit
CATCCATTTGATCAGGAGTCCTTTGAACACCATTTTATGACAATTCCATT11.6129032258064515No Hit
CTTGTCTACTGGCACGACGCCCTCTTGCTGGCCTAACTTAGAGAAGGCCT11.6129032258064515No Hit
GTATTCAAGTGATTAGGGTCTTTTGTTTGTAAATTGAAGAGCTATCTTTA11.6129032258064515No Hit
GGTCTATGTATTTGACTGTATTTTTTTTTTTTTTTTTGGAGATGGAGTCT11.6129032258064515No Hit
CTCTCGCCCTCTCAAATTAGCAAAGAAGGAAACCTCCCAGGAGATGAGAG11.6129032258064515No Hit
CGCTCATCCAATTCTTTTTTTTTTTTTTTTGAGATGGAGTCTCGCCTTGT11.6129032258064515No Hit
GCCCAAATAAGTCTTTTGAGTATTTCTAGTAAATTTTACATTTTGGTTAT11.6129032258064515No Hit
TCCACTCTTTTCAACCGTCACCAAAAACCACTCTAACTGCAGACAAAACG11.6129032258064515No Hit
CCTCAGATCTGCCTTCAAGGATAGAGCGTGACTACCTGTCTCTTATACAT11.6129032258064515No Hit
CCTTTCAGTAGGATACTCACGCCCATCATACAGTGCAGGGTCATGCAGAT11.6129032258064515No Hit
CCTTTGGGTGCATATTCTTAGGCTTTTTTTTTTTTTTATTCAAAAACAAT11.6129032258064515No Hit
GATTATAGACGCCCGCCACCATGCCCGGATAATTTTTTTTTTTTTTTTTT11.6129032258064515No Hit
CACCACAACTTTCACAGATCTGGAGGGTGCAAGGAGGAGCCATGGGAGAG11.6129032258064515No Hit
TAATACCCCCCCGCGTTGTCACCCCTCCCTGCTCCCTTCGCTGCGGAGCA11.6129032258064515No Hit
ACTTTATATGTTGGACATATGGTATGTGGCTTCCATTTATTTGTGCTTTT11.6129032258064515No Hit
CTCCGAAGGTCCTAGGATTACAGGAATCAGCCTGGCTGTTATTCATATCT11.6129032258064515No Hit
GCTTTCTGGAGTTTTAACAACAGCATCCATGTGAAGGGCCGGGCACATTT11.6129032258064515No Hit
ATCCTACTTTGCCAATTTATGGTTTTTTTTCTTTTTTTTTTTTTTTACAT11.6129032258064515No Hit
GATTAGCCCAAGCTAGGTTTTTCATGAAAATAACCTTATGGGCTGTCTCT11.6129032258064515No Hit
TCTGTCTGTTGGTATCAGAGAGTACTTTAGAGGGGAGATGGCCTGTCTCT11.6129032258064515No Hit
TATTAGCCCTTTGTCAGATGAATAGATTTCAAAATTTTTCTCCCATTCTG11.6129032258064515No Hit
ATTCTGAACTTTCCAATAAGCATTGACGATTAAAATTTTAGACAAAATTT11.6129032258064515No Hit
GTCTCTTTCCTTCCTTCCTTTTTTTTTTTTTTTTTTTTTTTTGAGACGCA11.6129032258064515No Hit
ACCCGCCCAACGTGGCTGGAGGTTGCACACCTACGAGCCATCTGCCAGCG11.6129032258064515No Hit
TCTAGAACCACTGCTCAAAACCCCATAGCTGAGATTTCCAAAGCTTCAAC11.6129032258064515No Hit
GGCTTATTTCACACTGCAGGAATCAAATCAGTACAGCTAATCATCAAGTT11.6129032258064515No Hit
ATTCAGGAATCATCTGTTGACTTTACTGTAGTTACTCATAATTTCTTTTT11.6129032258064515No Hit
GTACACAAATGCTAATGTTTTTTTTTTTTTTCCTTTTAGGCTCTGATGAT11.6129032258064515No Hit
CTCTTAACTAGAATTCTCATCTTGCCTTCTTTGATTTAATTCTCTGCCAT11.6129032258064515No Hit
TATTTGTATAGTGTTAAGGGTTCCTGTTGGAATTAATTTCCAATTTTATT11.6129032258064515No Hit
CATGTAGGTCTCTACGTAGCATCACCCCTAAACCTTGCATCCAGACCCAA11.6129032258064515No Hit
TTACAGGAGTGAGCCACCGTACCTGGCCTCTTTTTTTTTTTTTTTTTTAA11.6129032258064515No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGGA50.0145.02
GAGTGAG50.0145.07
TTACAGG50.0145.01
GTGAGCC50.0145.09
GCTGCCC50.0145.0145
AGTGAGC50.0145.08
ACAGGAG50.0145.03
CAGGAGT50.0145.04
AGGAGTG50.0145.05
GGAGTGA50.0145.06
CCCCCCC350.00429605816.571428110-114
TTTTTTT600.012.08333435-39
Produced by FastQC (version 0.11.5)