FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n02_MG_341.tr1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n02_MG_341.tr1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7372966
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT29500.033.665329
TCTCGGG30950.031.15102145
CTCGGAG30050.029.6714637
GCTCGGA22250.029.3218146
TCGGAGA30150.028.3712798
GACGAAA46700.025.45706145
CGGTGGG45100.020.413076145
TACACCG24150.017.7101425
TCGCCGG63900.017.016558145
TGACGCC28100.015.736378145
GGCTCGG24300.015.5125875
TACACAG59150.015.1968675
GTGCTAG32600.014.6759981
GTCCTAG26900.014.5520031
TACACTG48000.014.49830155
TATACCG14650.014.3498555
CTACACT40100.014.2812754
CCTACAC33600.014.2393073
GTATAGG30600.014.213831
CTATACT45250.014.0977024
Produced by FastQC (version 0.11.5)