FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n02_MG_3256.tr4-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n02_MG_3256.tr4-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9725205
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT33850.075.167339
TCGGAGA29800.071.030898
GCTCGGA22500.067.334866
CTCGGAG37150.058.3435757
TGACGCC25100.042.74336145
ACGATCC23250.041.77943145
GGCTCGG24250.038.8628465
TTACGCC17450.034.06427145
GGGCTCG18700.031.4010524
TACGCGG6650.030.522285145
GCCGACC19000.030.140755145
CGACGAA128700.026.472805145
TAGCTCG8950.025.9195634
ACTCGGA16300.024.4597076
ACGCGGA5850.023.5436576
GCGTGTT70950.022.579823145
TGCTCGG19500.021.934135
CGCGGAG10300.021.8175097
GCACGGA12550.017.9058176
ACGAACC16900.017.586435145
Produced by FastQC (version 0.11.5)