FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n02_MG_30982.tr4-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n02_MG_30982.tr4-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6682207
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAG24450.035.2813727
CGGAGAT31000.032.5042579
TCGGAGA28400.029.3539568
GCTCGGA24100.027.9732726
TCTCGGG32000.021.520918145
TACACTG44850.017.4568025
TATACTG52550.016.8302465
CGTACAC17150.015.6401523
CTATACT35600.015.4762944
TCTATAC38050.015.4324093
TGACGCC42200.015.288493145
GTATACG11450.015.1951811
TACACCG22550.015.109665
GTATAGG24900.014.8481031
GTATAGA40150.014.8056821
GTATAGC29000.014.4987361
CCTATAC24550.014.1740223
CTACACT34500.014.0785874
TAAGGGT30900.014.0765484
GTACACT26150.013.8612274
Produced by FastQC (version 0.11.5)