FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n02_MG_30900.tr3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n02_MG_30900.tr3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTCAGCCTCCCAAGTAGCTGGGATTACTAGCATGCACCACCACGCCTG12.7027027027027026No Hit
ATTCTAAAGGGCATCCTGCATGATTCACAGAGGCCCCAATGAGATGGAGT12.7027027027027026No Hit
CCACAAGGATGTGTATGCCACTCCACCCCCAGCTTTAGGTGGGTCAGAAC12.7027027027027026No Hit
GCAAAATACCAGCGAAACAGCAGGCACTGAATTGATACACAGAATTCTTT12.7027027027027026No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTTTTTTTTTTTTGAGATAGTGTC12.7027027027027026No Hit
CCCCAGGACTTGGTGGTATGTCCCCTCTTACTTTTTTTTTTTTTTTCGGT12.7027027027027026No Hit
GCACTAGGATTACAGGCGTGAGCCCCTGCCCCCAGCCCCTCAGAAAGCCT12.7027027027027026No Hit
TACCAAACTTTACTGGTAATTACCTTTCAGTGCTAGATTTTTCAAGCCGT12.7027027027027026No Hit
GTTGTCAACACATATATATACACTACACACTTTTTTTTTTTTTTTTGGTG12.7027027027027026No Hit
CGTGGCAGAGTGGTGAGGGACTGGGACGACGAATGGGAGGAGGGATGCTA12.7027027027027026No Hit
CCTGTGACCGGAGAGGTCACATCTCGCCATCGGCATCCACGTGGCGGCCA12.7027027027027026No Hit
CTTCTACTCAGAATGTTCTCCCACATGTCTTCCAGTCAAAAGCCTACTCT12.7027027027027026No Hit
GAGTACCACTAGTATTTAATAGAAACTTTTTCTGTCTCACAGGGAACTAT12.7027027027027026No Hit
GTATGCATTATGAAATACTGCAATGCAACTCTTTTTTTTTTTTTTTTTTT12.7027027027027026No Hit
ATTGTATCTTTCCATATTCTGATGGCTTTTGTGTCATTCAAAGATAAGTT12.7027027027027026No Hit
TACACACACACACGTCACGAGCTTGATCAAAGATGGACGGGACACAAATC12.7027027027027026No Hit
TGATTACATAGTGTAAGCAAGTAAACCTTTGCTTTAAAGCACTGAGCTTT12.7027027027027026No Hit
AAAAATCTGTACCGTCATTCCAAAAACTGGTCCGAGTATCCGAAACAGCT12.7027027027027026No Hit
CTCCTGAGTAGCTAGGATAACAGGGACCCGCCACCATGCCTGGCTAATTT12.7027027027027026No Hit
ACTCTGTTGATAGCCCTCAGTGTTTTTTTTTTTTTTTTTTTTTTTTACGC12.7027027027027026No Hit
GTTTTCCTCTGCTTCAGTTTGCTTTCATAAGAAACATGAGATCTATTTCT12.7027027027027026No Hit
GTCTAGGAATGGGTAACCCAGCCCCATCCATTGATAAGTGCTTATGAGGG12.7027027027027026No Hit
ACTTTTTATAGTTTTTTTTTTTAACTAGGAAAAAAATACATCAAAATGTC12.7027027027027026No Hit
AAGCTATGCTGTGACTGGGTTGTGGCAATTTCATGATAACACTTGAACAA12.7027027027027026No Hit
ATATTGTTCTGTGTTTTTGTTTTCTTTTTTTTTTTTTGATGCTATTGTAT12.7027027027027026No Hit
CTTTTAGGTTTTTTTTTTTAAATAGAGACAGGGTCTTACTATGTTGCGTA12.7027027027027026No Hit
TGCCAGGGCTGGTACTATTTTTTTTTTTTTTCCTGAATAATTTTGGACTC12.7027027027027026No Hit
CTTGGGGACGACCAGCCTCAAGCGCCGTAATACAATATCTTTTCTGGCTG12.7027027027027026No Hit
CTTTTGTCTTAATGACTGTTAATTCTTTCTCTAAGCTTATTGAGTAGTGT12.7027027027027026No Hit
AATCAAATATTTATGCAATAAAATTAAGCAATAATATGTTCTTTAATTAC12.7027027027027026No Hit
TATTAATACATTGTATGAATATAATACAGTTTATTTGTCCTATCTCCTAT12.7027027027027026No Hit
CCCAGGTAATTTTTTTTTTTTTTTTTTTTTTTTTTGTAGAGAGGGGGTTT12.7027027027027026No Hit
GCCGACAGGAAGTCAGGATCCTCCCAAAACATCCCGGGCTATAACCGTCG12.7027027027027026No Hit
ATTCAAATATTTTGTCCTTTTTTTTTTTTTTTAGAGGGGGTCCCCCCCTG12.7027027027027026No Hit
GTGTGAGGAAGGGGTCCAGTTTTAATNNNNNNNNNNNNNNNNNNNNNNNN12.7027027027027026No Hit
GCACATTGGCTTTGTTTTTGGTCATGACCTGTTGAAGCCTGGAAAGATCC12.7027027027027026No Hit
CAGCTAGAGAGTGATGTAGCCAGGGTTGTTTGAAACTAGCAGTCTGCCAT12.7027027027027026No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)