FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n02_MG_30900.tr3-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n02_MG_30900.tr3-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10364716
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAT35350.033.8361479
GCTCGGA24900.032.0235336
TCGGAGA36450.027.44538
CTCGGAG40050.026.6070697
TGACGCC38150.024.701939145
CGACGAA123950.024.621687145
ACGATCC31100.023.075813145
GCGTGTT70200.018.897144145
GGCTCGG31100.016.3164675
TACACTG76800.015.4800055
GCCGACC34850.015.184563145
TATACTG75500.015.074445
ACGCGTT129100.014.374606145
TTACGCC27600.013.657665145
CTATACT62100.013.4243314
CTACACT61000.013.3098944
TACACCG20350.013.1803165
CTAGGAC41050.013.0678753
GTCTTAG55050.012.9047921
CCTACAC51300.012.8590973
Produced by FastQC (version 0.11.5)