FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5684693
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG229180.40315281757519716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA2600.055.5274246
CTCGGAG2800.051.550757
GGGCTCG2950.048.939434
GGCTCGG3900.037.0182845
TCGGAGA3900.037.0107968
CGGAGAT4050.035.6400269
CGTGGGC4450.032.455271
GTGGGCT6000.024.0640032
TATACAG4902.0008883E-1120.6244745
CCCTAGG2506.1799525E-520.2137622
ATATAGG3601.2288365E-720.0591581
GACCGAA3351.2463297E-619.589455145
TGGGCTC7550.019.122033
GCACTGA5005.7661964E-1018.768276
GCCTACG2359.455936E-418.4373551
GTATAGA4506.601476E-817.652061
TTGTACT5751.9645086E-1017.5756384
GGACCAT2500.001435671417.3245586
ACCGCGT2500.001437618417.3210538
TGTACTG4659.640644E-817.0761785
Produced by FastQC (version 0.11.5)