FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n01_MG_positive-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n01_MG_positive-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8346682
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACT23150.018.4796184
TATACCG40800.018.127255
CTATACT26800.017.0450274
GTGTAGG31350.016.4249421
GTATAGA31800.016.4205781
CCTATAC26300.016.266283
TAGGACA20550.016.2307264
GTCCTAG10750.015.51680851
CTATACC36850.015.3479034
TCTATAC30350.015.2902023
CTACACA22400.015.2139434
CTTACAC29300.015.0957323
TACACTG32450.014.9710445
GTCTAGG12150.014.9226781
TATACTG40100.014.8272785
TATACAG49300.014.5606335
GTCCTAT17650.014.3815731
TAGACCC16650.014.3711544
TCTACAC34350.014.1429493
GTGTAGA34550.013.8541191
Produced by FastQC (version 0.11.5)