FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n01_MG_neg.8.7-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n01_MG_neg.8.7-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13402
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT367427.41381883301No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC4103.0592448888225636No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG3642.7160125354424713No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT1190.8879271750485002RNA PCR Primer, Index 39 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCC600.4476943739740337No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCT300.22384718698701686RNA PCR Primer, Index 39 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT290.21638561408744966RNA PCR Primer, Index 39 (95% over 22bp)
CCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG280.2089240411878824No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACAACTCGTATGCCGT220.16415460379047903No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACACCTCGTATGCCGT180.13430831219221012No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT160.11938516639307566RNA PCR Primer, Index 39 (96% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACAGCTCGTATGCCGT150.11192359349350843No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC140.1044620205939412No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG100.007013535145.06
TCCGGTT100.007013535145.04
GAACAGA100.007013535145.0145
AAAGATC100.007013535145.0145
CATCGTG100.007013535145.07
TTATACA7200.092.6388852
CTTATAC7200.092.6388851
TATACAC7350.090.74833
CACATCT7400.090.135137
TACACAT7400.090.135135
CATCTCC7450.089.53029
ACACATC7550.088.344376
ATACACA7700.086.623384
CGCATCG400.00592193754.3755
ACATCTC13750.048.5090948
CTCTTAT2050.042.4390261
TCTTATA2501.8189894E-1234.82
CTTGAAA6550.024.35114555-59
TCTTCTG6500.024.31538650-54
ATCTCGT6500.024.09230835-39
Produced by FastQC (version 0.11.5)