FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n01_MG_neg.8.2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n01_MG_neg.8.2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2621
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT134551.31629149179703No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC1154.38763830599008No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG1114.235024799694774No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT401.5261350629530714TruSeq Adapter, Index 10 (95% over 21bp)
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT140.534147272033575No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCCGT130.49599389545974815No Hit
CCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG110.4196871423120946No Hit
CTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAGCC100.38153376573826786No Hit
CTTATACACATCTCCGAGCACACGAGACCGAGGCTGATCTCGTATGCCGT90.3433803891644411No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGACCTCGTATGCCGT70.2670736360167875No Hit
CTTTACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC60.22892025944296068No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAGCTCGTATGCCGT60.22892025944296068No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT60.22892025944296068TruSeq Adapter, Index 10 (96% over 26bp)
CGTGGGCTCGGAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCC50.19076688286913393No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG40.15261350629530715TruSeq Adapter, Index 10 (95% over 24bp)
CTCTTATACACATCTCCGAGCCCACGCTGTCTCTTATACACATCTCCGAG40.15261350629530715No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGATGATCTCGTATGCCGT40.15261350629530715No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC40.15261350629530715No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATATCGTATGCCGT40.15261350629530715No Hit
CTTATACACATCTCCGAGCCCACGGACCGAGGCTGATCTCGTATGCCGTC40.15261350629530715No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG40.15261350629530715No Hit
GTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT30.11446012972148034No Hit
CTTATACACATCTCCGAGCCCACGAGAACGAGGCTGATCTCGTATGCCGT30.11446012972148034No Hit
TCTTATACACATCTCCGAGCCCACAGACCGAGGCTGATCTCGTATGCCGT30.11446012972148034No Hit
CTTATACACATCTCCGAGACCACGAGACCGAGGCTGATCTCGTATGCCGT30.11446012972148034No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTATCTCGTATGCCG30.11446012972148034No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGAGGCTGAACTCGTATGCC30.11446012972148034No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTAATCTCGTATGCCGT30.11446012972148034No Hit
ATACACATCTCCGAGCACACGAGACCGAGGCTGATCTCGTATGCCGTCTT30.11446012972148034Illumina PCR Primer Index 10 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACATCT2050.0113.170737
TTATACA2050.0113.170732
ACACATC2050.0113.170736
CTTATAC2050.0113.170731
TATACAC2100.0110.476193
CATCTCC2100.0110.476199
ACATCTC2100.0110.476198
TACACAT2100.0110.476195
ATACACA2200.0105.4545444
CTCTTAT459.846347E-564.444441
TCTTATA501.6588505E-458.02
GTATGCC1950.027.51282140-44
TGCCGTC1950.027.51282145-49
TTCTGCT1950.027.51282150-54
TGCTTGA1950.027.51282155-59
CGTATGC1950.027.51282140-44
GCTTGAA1950.027.51282155-59
TCGTATG1950.027.51282140-44
GCCGTCT1950.027.51282145-49
CTTGAAA1950.027.51282155-59
Produced by FastQC (version 0.11.5)