FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n01_MG_8724.tr6-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n01_MG_8724.tr6-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8998923
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAAG43550.017.1543251
ACGGACC24000.016.311712145
TATACTG67250.015.8499885
GTATTAG56900.015.55149651
GCTCGGA15850.015.0969286
TATACCG16750.014.285755
GTATAGA51550.014.2107591
TACACTG54300.014.0214645
GTGTTAG48650.014.0142421
GTGTAGA51550.013.92935851
TACACCG22400.013.9195225
TATACAG62300.013.8504165
GGACCGT13200.013.7331456
GTATAAG52850.013.7239661
CTAGTAC25400.013.7029433
GTGTAGG36650.013.65525151
TATAGTG45100.013.1838195
CTATACT47350.013.1698984
CTAGCGT9408.0035534E-1113.1136914
TAGACTG44450.012.887225
Produced by FastQC (version 0.11.5)