FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n01_MG_8724.tr3-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n01_MG_8724.tr3-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9880224
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGCC33300.026.125137145
GCTCGGA13500.023.0962776
TCGGAGA16500.018.8932238
GGGCTCG16300.017.794254
GGCTCGG17150.016.912325
CGGAGAT18750.016.6260389
TACACCG12100.016.180275
TACACTG74900.016.1674675
TATACTG83800.016.0944525
GTATTAG61850.016.0665361
CTACACT55100.015.7919934
GTGTAGG39550.015.772241
CTCGGAG19500.015.6147947
CTATACT57150.014.5911294
GTCGTAC7002.9776857E-914.5067931
TCTACAC57150.014.337373
GTCTAGC37500.014.1199451
GTATACG13900.014.0893311
GTGTAAG53100.013.9330771
CCTACAC43650.013.7879953
Produced by FastQC (version 0.11.5)