FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n01_MG_8337.tr2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n01_MG_8337.tr2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10910359
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG96700.017.1714045
GCTCGGA12000.016.3147096
TACACCG9450.016.1132935
TCTACAC61400.015.5884313
GTGTAAG64800.015.1105761
GTGTAGA56100.014.9974471
CTATACT63450.014.7419494
TAATACT100600.014.6317034
TACACTG79900.014.4293635
GTATAGG34850.014.1523441
GTGTTAG76900.014.1477441
CTACACT60550.014.13073354
CTAGGAC48250.013.82570653
GTATTAG63850.013.7450631
CTTACAC63900.013.7303423
GACTGCG11200.013.5931627
TAGACAG127200.013.5670895
TACACAG100100.013.4733525
TTATACT110200.013.422874
ACACGGT33100.013.3628376
Produced by FastQC (version 0.11.5)