FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n01_MG_7784.tr2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n01_MG_7784.tr2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9396787
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG24250.014.9507565
TATACTG70150.014.3678585
GGACCGT14650.014.3537476
GTGTAGG43200.014.2717261
TACACTG61350.014.1831135
GTCTTAC48450.013.7732151
TAGACAG87150.013.7284495
GTATATG77300.013.6998151
GTATTAG59450.013.5429241
CCTAGAC30650.013.4849483
GTATAGA58450.013.4023371
GTCCTAG28300.013.3277921
GTGTAGA57900.013.27909851
TAGGACA47400.013.1560354
CTAGACA73600.012.9061994
GTGCTAG32700.012.8653481
TAATACT94750.012.8568624
CTACACT47450.012.8365394
GTATTGG57300.012.7852151
CTAGACT33550.012.7515924
Produced by FastQC (version 0.11.5)