FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n01_MG_7379.tr2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n01_MG_7379.tr2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9955085
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAGA21100.029.2051778
GCTCGGA21400.028.8014016
CGGAGAT24900.024.7481639
GGGCTCG25500.024.1705884
CTCGGAG30250.020.3712147
GGCTCGG31700.019.671965
TAACACG34900.018.4915394
TACACTG65100.016.2622385
TACACCG12100.016.180315
TATACTG48950.016.1466435
CGTGGGC40200.015.516831
GTATTAG47400.015.4551661
GTATAAG41300.014.5766821
CTACACT59700.014.575234
ACACGGT47000.014.5023516
GTATAGA39050.014.487861
CTATACT41450.014.3449094
AACACGG48700.014.1450045
TACAGGT181650.013.8516824
GTCGTAC6402.282286E-713.5997811
Produced by FastQC (version 0.11.5)