FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n01_MG_341.tr1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n01_MG_341.tr1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCCAACGCCACACAGAACAGCCCCAGCCTCGCCAAGCTCCCCAACAGCG11.6129032258064515No Hit
AGCTTGCAGTGAGCCAAGATTGCGGCACTGCACTCCAGCCTGGGCGACAG11.6129032258064515No Hit
GGCTGAGGCAGAATAATCTATTGAACCCAGGAGGCGGAGGTTGCAGTGAG11.6129032258064515No Hit
GTGAGACTCCATCTCCAAAAAAAAAAAAAAAAATAACGTCAAAAACAAAC11.6129032258064515No Hit
ACTATAACCAAAACCCATAGATGCATTAAAAACAAAACAACCAACAAAAA11.6129032258064515No Hit
GCCGTATGTTGTTGTCAGCTTGAAATTATCAGTTTGTGACAATTTTAGGA11.6129032258064515No Hit
CGCTATTCGTCTCCTAGCCAACACAATTCTCATGACATTATAGCTGTCTC11.6129032258064515No Hit
GGCCAGGTGTGGTGGCTCAAGCCTGTAATCCCAGCACTTTGGGAGGCCGA11.6129032258064515No Hit
GTATACCTATGTAACAAACCTGTATGTTTTGCACATGTACCCCAGAACTT11.6129032258064515No Hit
GTTCTAACTCAGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC11.6129032258064515Illumina PCR Primer Index 5 (95% over 21bp)
ACTCCAGCCTGGGCAACAAGAGCAAAACTCTGTCTCAAAAAAAAAAAAAA11.6129032258064515No Hit
CCATCACACTGGGGGTTAGGACTTCAAAGTATGTATTTGGGAGGGACACA11.6129032258064515No Hit
GGCTATGGCAGGAGAATCGCTTGAACCTGTGAGGTGGAGGTTGTGGTGAG11.6129032258064515No Hit
GGATAAATCAAACTGGTATAGGAATGCCTACAATAAATGGAGAACATGAA11.6129032258064515No Hit
ACCTTATATTTCTTCAATGCACTTATGTAGGGTGTGGAAAAAAAAAAAAA11.6129032258064515No Hit
CGTGGGCTCGGAGATGTGTATAAGAGACAGGCTCATGCCTGTAATCCTAG11.6129032258064515No Hit
GACATAGGGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTCTGC11.6129032258064515RNA PCR Primer, Index 8 (96% over 26bp)
GCATGGCTTTGTAAAAACCATTCAAAATGTATGACAAGTATCAAAAAATG11.6129032258064515No Hit
GTGGCGGGCGCCTATAATCCCAGCTACTCGGGAGGCTGAAGCAGGAGAAT11.6129032258064515No Hit
CTTCAACAAGAGTAGTTATCTTTGTATCTCCAGACATAGCTCAGTGTTTG11.6129032258064515No Hit
GTGAGCAGTCCACAGAGCAGAGCCCACGAGACTAGGCATGATCTCGTATG11.6129032258064515No Hit
GTGGTACACAACTGTAGTCCCAGCTACTTGGGAGGCTGAGGCAGAAGAAT11.6129032258064515No Hit
AAACAAAAACAGCTCTCATGATAACATCTAAAAATGAAGATAAAGTGACA11.6129032258064515No Hit
GATTCAGAAAGGTAAAAAAAGAGGTCAGAGGCCAGGCACAGTGGCTCATG11.6129032258064515No Hit
GAGGAACTTGATGATTAGCTGTACTGATTTGATTCCTTCAGTGTGAAATA11.6129032258064515No Hit
GCCATCTCCCCTCTAAAGTACTCTCTGATACCAACAGACAGACTGTCTCT11.6129032258064515No Hit
AGCCTGGCCAACATGGAGAAACCCTGTCTCTACTAAAAATACAAAAAAAA11.6129032258064515No Hit
CTTCTGCACAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.6129032258064515No Hit
GTCATAGGCATGACCTGAAGAGAAGCAGCTGTGCTTTGTTTTTTGCTAGG11.6129032258064515No Hit
TACAAATGCCAACTCGTATAATTATCAGAAAAGCCAATTTAGTAACCAGA11.6129032258064515No Hit
GCTACTACGTGGGCGAGAGCGACGGCACGGTGGCGGTGTACAACGGTATT11.6129032258064515No Hit
AGGTTGCAGTGAGCCGAGACCATGCCACCGCACTCCAGCCAGGGCAACAA11.6129032258064515No Hit
GGTTGCTGACAGCAGGAACACATTTTCCACTTTTATTTGCATGTGGGTTA11.6129032258064515No Hit
CCCATAAGGTTATTTTCATGAAAAACCTAGCTTGGGCTAATCCTGTCTCT11.6129032258064515No Hit
ATTCCTTGCAATGCATCGGAAACAACTAAGTCTATCTGCTCGACGCCTCT11.6129032258064515No Hit
TACAAAAATTAGCTGGGTGTTGTGGTGTGCACCTGTAGTCCCAGCTACTT11.6129032258064515No Hit
GGGAGGTGGAGGTTGCAGTGAGCCGAGATCGCGCCACTGCACTCCAGCTT11.6129032258064515No Hit
GAGACAACACTGACTGAGGAAATAGGCTAAATAGTTTGGGGCTTACTCCG11.6129032258064515No Hit
GTTGAGATTCTTTTCATGTGCTTGCTAGCCATGTGTACATCTTTGTTGGA11.6129032258064515No Hit
GTGTGAACCCAGAGAGTCATGGGGAAGCATTGCAGAAATACTTGAAGCAA11.6129032258064515No Hit
CTGCTATTGTTTTAGTAAACGAATGCTTAAACTGACAGAAAGTAAAAAAT11.6129032258064515No Hit
ACTTAAAAGATACAGAACTGGCCTGGCATGGTGGCTCATGCCTGTAATCC11.6129032258064515No Hit
ACCCCAAGTCTGCTCCTAGGCTGCTGTTCCTTTTAATTACTGCCTAGCAG11.6129032258064515No Hit
CTACAATGCTGATATCACCTACGGCACCAACAACGAGTTCGGATTCGACT11.6129032258064515No Hit
GATTATTCCATTCGAGTCCATTCGATGATTCCATTCGATTCCATTCGATG11.6129032258064515No Hit
GGTGAATCACCTGAGGTCGGGAGTTCGAGACCACCCTGGCTAAGATGGTG11.6129032258064515No Hit
GGCGTGAACCCAGGAGGTGGAGCTTGCAGTGAGCTGAGATCGCACCACTG11.6129032258064515No Hit
GCAATAGATAATTTATAAGTATATGCTGCCTCTTGCTGCAACACTTACTT11.6129032258064515No Hit
GCCTAGAACTGCAAATTCTTAGGCCCCACCCAAGACCTATTGAATAGAAA11.6129032258064515No Hit
CTTGAAATCATGTCCTCAAATGATCTTCCCACCTTGCGCCTGGTGCCGTG11.6129032258064515No Hit
GGCCAAGGATCCTAGAATAGAAAAGAGGCAGGATGCACAGCGACCTTAGC11.6129032258064515No Hit
GAATAAAATAATAGAAAACTTTACTTACTATTCTGTAGTCTAGTTTCTGG11.6129032258064515No Hit
CCCTCCTGACCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC11.6129032258064515Illumina PCR Primer Index 5 (95% over 21bp)
ACTCGGGAGGCTGAGGCAGGAGAATTGCTTGAACCTAGGAGGTGGAGGTT11.6129032258064515No Hit
GTAGTAACGCTCTATCCTTGAAGGCAGAACTGAGGCTGTCTCTTATACAC11.6129032258064515No Hit
ATATAGAGGTAATGCCTTCCAGGGGCTCTCAAGCTAGTGGCTTTATCTGT11.6129032258064515No Hit
GTAATAAGTAATGCTATCAATTACATCCTCTTGCCTCAATGCTCAAATAT11.6129032258064515No Hit
AGGTAGGAGAATCACTTGAACCTGCGAGGCAGAGGTTGCAGTGAGCCAAG11.6129032258064515No Hit
CTCATGGACAGTGGTCATCTGCCATACTTTTCCTCCTCCCCTGGTCCTCT11.6129032258064515No Hit
GTTTGTGCCTGTTGTCCCAGCTACTTCAGAATCTGAGGCAGGAGGATCCC11.6129032258064515No Hit
ACTCAAGCCTTCCCGAAGAGTATCTAAAATGAAGTTTATGGTATACCCAT11.6129032258064515No Hit
ATCCTGAGCTCATATTATGTTTTAAGCTTAATTGAGGAATAATTGCTGTC11.6129032258064515No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC50.0145.0145
GAGGTGG50.0145.03
TGGAGGT50.0145.07
GGGAGGT50.0145.01
AGGTGGA50.0145.04
GGAGGTT50.0145.08
GGAGGTG50.0145.02
GGTGGAG50.0145.05
GTGGAGG50.0145.06
GAGGTTG50.0145.09
AAAAAAA600.012.08333475-79
Produced by FastQC (version 0.11.5)