FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n01_MG_341.tr1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n01_MG_341.tr1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7372966
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC30800.016.479783
TACACTG45850.016.2892825
TATACTG65200.015.9034935
CTACACT40300.015.4738174
GTGTAGG35450.014.7314021
GTATAGA46750.014.4287641
CTATACT47300.014.4102264
TACACTC36600.014.2644645
TCTACAC46050.014.1715373
TACACCG24200.014.0827215
TCTATAC49500.013.9162583
TACACAG59550.013.8812055
GTGTAAG40000.013.7810221
GTCTAAG30750.013.6807741
CTATACA49100.013.4389094
GTGTATA67050.013.305591
GTACTAG20850.013.2192061
GTATAGG25600.013.0330381
GTCCTAG26800.012.990751
GTGTAGA44900.012.9232421
Produced by FastQC (version 0.11.5)