FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n01_MG_3256.tr4-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n01_MG_3256.tr4-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9725205
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA16300.039.5923926
GGGCTCG17200.034.9912154
CGGAGAT18400.032.7022829
TCGGAGA19000.032.43278
GGCTCGG19550.031.5269175
CTCGGAG20800.029.2774837
AGACGCC24400.027.037676145
CGTGGGC25150.024.2264141
TATACTG98950.016.5615885
GTGTAGG43650.015.45421
TACACTG79800.014.6295955
TAGGGTG44800.013.9196985
GCTAGAC39900.013.8118633
GTCTACG7951.2114469E-913.6858551
GTCCTAG43500.013.1730291
GTCTAAG44150.013.143381
CTATACT64050.013.1325244
TCCGTAC9951.4551915E-1113.11783
GTATTAC50000.013.0563061
GTATTAG61400.012.876741
Produced by FastQC (version 0.11.5)