FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n01_MG_30982.tr4-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n01_MG_30982.tr4-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6682207
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG16450.017.191025
CTAGGAC22550.017.0424583
CCTACAC30850.016.2179833
GTGTAGG26600.016.0874441
GTATAGA38000.016.032911
CTCGGAG19500.015.6145627
TATACTG50350.015.2654385
CTACACC37950.015.0944814
TACACCG21900.014.5683865
TACACTG43550.014.3190165
GCTCGGA21850.014.2698666
CTACACT33700.014.2009344
CCTATAC23350.013.974253
CTAATAC40100.013.9235273
TCGGAGA21900.013.5723458
GTGTAGT31000.013.3361291
GTATTAG38950.013.2210991
TCTACAC36200.013.01993
GTATAGG24150.012.9142151
TAAGGGT30400.012.8802134
Produced by FastQC (version 0.11.5)