FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n01_MG_30900.tr3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n01_MG_30900.tr3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACCCTAATAACAAAACCATACAATAGCATCAAAAAAAAAAAAAGA12.7027027027027026No Hit
GAGGTGGAGGTTTCAGTGAGCCGAGATCATGCCACTGCACTCCAACCAGG12.7027027027027026No Hit
AGCAAGAAGTAAGCCACAATGTAACTTAGAAGTTATAAACCATTGTTTCT12.7027027027027026No Hit
CCAGTAACCATATTCTGCATCTCTTTTTCCTCAGTGCGATACACTTGCCC12.7027027027027026No Hit
CCTGTAATCTCAGCACTTTGGGAGGCCAAGCCAGGTGGATCACAAGGTGA12.7027027027027026No Hit
CCCCATCTCTACTAAAAATGCAAAATTAGCCGGATGTGGTGGCACATGCC12.7027027027027026No Hit
CGTGGGCTCGGAGATGTGTATAAGAGACAGGTTATTGAGACCTAACAAAA12.7027027027027026No Hit
GAACTACAGTCCACTCCCTACCTCTGTTAAATCTTTACTTAAGGCCAGAT12.7027027027027026No Hit
GGGGGGGGGGGGGGGGCGGGGGGGGGGGGAGGGGGAGGGGGGGGAGGGGG12.7027027027027026No Hit
CCACACATTCAATTGCGTAAAAAAAAAAAAAAAAAAAAAAACAACAGGGG12.7027027027027026No Hit
CTAATTAGCTGGGTGTTGTAGCGCACACCCGGTAATCCCAGCTACTCGGG12.7027027027027026No Hit
TCTCATGCCTGTAATCCCAGCACTTTGGGAGGCTGAGGCGGGTGGATCAC12.7027027027027026No Hit
GCTTCTCCCATCTGCCACCTTAGCAAGCAAAAGAGTTCAGCTTGAAATTT12.7027027027027026No Hit
CGTGGGCTCGGAGATGTGTATAAGAGACAGCTGTGGCCCAGGCTGGAGTG12.7027027027027026No Hit
GGGTGGATCATCTGAGGTGGGGAGTTCGAGACCAGCCTGATCAACATGAA12.7027027027027026No Hit
GGCCAAGGCGGGCGGATCACAAGGTCAGGAGTTCAAGACCAGCCTGACCA12.7027027027027026No Hit
GGCCAAGAGGGAGGGGCAGAAAACAATAAGCATGGGAAACAGTACAAAAG12.7027027027027026No Hit
CACATTGCCTTCAAAATAAAGTCACAAAACAGTAGCAAACCTGGCTGGGC12.7027027027027026No Hit
GGATCATTGTCACTTTTCTCTTCCACCACATATAAGAAGGAAACAAATGA12.7027027027027026No Hit
GCACACGCCTGTAATCCCAGCTACTAGTGAGGCTGAGGCAGGGGAATTAC12.7027027027027026No Hit
GAGCGTGGTGGCGCGTGCCTGTAATCCCAGCTACTCGGGGGTCTGAGACA12.7027027027027026No Hit
GCTCACACTTCAGGCCGAACTTCTTCCCAATCTTGTTGATTCTGACCATA12.7027027027027026No Hit
CCCCATCTCTACTAAAAATACAAAATTAGCCAGGCGTGGTGGTGCATGCT12.7027027027027026No Hit
GGCTGAGGAAGGAGAATTCCTTGTACCCAGGAGGCGGAGGTTGCAGTGAG12.7027027027027026No Hit
GCACAATGCTTTCTTTTTAAAACCAGGCACAGTGGCTCATGACAGTAATC12.7027027027027026No Hit
GGACTTACCTTGGCGGATGAACGTTTGGCTGGTGTCCAGCAAGATGTCAC12.7027027027027026No Hit
TTGTGCCACCGCACTCCAGCCTAGACTGCGAGACTCCATCTCAAAAAAAA12.7027027027027026No Hit
TGTGAGTACTTGTGAAAAGACCTTGTTACTGGTGTGGTCAAGAAGGCTTT12.7027027027027026No Hit
ATGTTCTTCAGTGTCTGGGCATTGAAGAATTAGGTATTTATTGTAGTCTT12.7027027027027026No Hit
GGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCTGG12.7027027027027026No Hit
ACTCATATGTTCTAGTTTGGGGTTCCTCCCAAAAGCAGAACCAAAGACAA12.7027027027027026No Hit
CTCTTTTTATACAATCACTGAAGGGACATTTCAGAGCCCACTGAAGTCTA12.7027027027027026No Hit
CCTACAATCATGGCGGAAGGTGAAGGGCACCAGCAGGTCACATGGCAAGA12.7027027027027026No Hit
GAGCATCTCTTCATCCAGCTCAGTGCCCACGAGACCTCTCTACATCTCGT12.7027027027027026No Hit
GGGGGAAATTTGACCAAAGTTCACAAACATGTACTAGATGGTCCTGAGAC12.7027027027027026No Hit
CGTGGGCTCGGAGATGTGTATAAGAGACAGGCTCTGGAGGTCAGACACTG12.7027027027027026No Hit
ACTCAGGAGGTTGAGGCAGGAGAATTGCTTGAACCTGGGAGGCGGAGGTT12.7027027027027026No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)