FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l02_n01_MG_30900.tr3-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l02_n01_MG_30900.tr3-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10364716
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG22850.016.5010155
TATACCG15250.014.7395915
GTATTAG69150.014.6838681
CCTACAC50500.014.5018543
TACACTG74550.014.200345
CTAGTAC31900.014.09271053
GTATAGG39650.013.71900751
CTACACT63250.013.4127824
GTGTTAG71500.013.0854391
GTGTAGG51800.013.0214041
GCTCGGA20050.013.0191216
GTGCTAG53200.012.9513961
TATACTG72950.012.7226695
TCTATAC61800.012.4368653
ATACTGC65900.012.4333046
TAATACT100600.012.3972114
GTATAAG64350.012.285211
TATACAG85200.012.2550895
CTAGGAC42100.012.0561743
CTTACAC60600.011.9652253
Produced by FastQC (version 0.11.5)