FastQCFastQC Report
Thu 11 Oct 2018
HK77CBCX2_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK77CBCX2_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5924519
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT11150.036.3517952
CTCGGAG4500.027.3565967
TCCCTAC6100.027.3218549
GCTCGGA3607.2759576E-1226.1490236
TTCCCTA7200.025.1594588
CGGAGAT5850.024.773449
TCTCGGG8600.023.771177145
TCAAGCC7500.023.36367145
TCCGATT3301.1584107E-619.7395671
CTAATAC4202.9151124E-818.9586563
TCGGAGA5407.6397555E-1118.785738
GTTCCGT2751.255048E-418.432326
TAGGACA4806.7884685E-918.1025584
GTCCTAC2400.001072563218.0946041
CTAGGCC3302.5490366E-517.5485063
TTGTACT5051.2720193E-817.2063924
TTTCCCT14100.016.948087
ACTGTAT6205.4205884E-1016.3617638
CGGTGGG15250.016.277967145
ATAGGAC3605.3746226E-516.086133
Produced by FastQC (version 0.11.5)